162,160 results

1. FAB(ER-mito)

   Plasmid

   #214427

   Purpose

   Detection of ER-mito contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   FRB-V5
   

   Use

   Tags

   CFAST10-Halo and RspAN-FRB-V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS1096 Dual-sgRNA.Design 1

   Plasmid

   #159538

   Purpose

   Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

   Depositor

   Erik Sontheimer

   Article

   Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

   Insert

   Nme2Cas9 nuclease with two guide RNA cassettes with promoters
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSelAct-KO

   Plasmid

   #174374

   Purpose

   Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Vector for 2-step knockout by homologous recombin…

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-mCherry

   Plasmid

   #92202

   Purpose

   Fluorescent protein reporter construct

   Depositor

   Alice Ting

   Article

   Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-Neo-ISG15-LRAA

   Plasmid

   #80405

   Purpose

   overexpression of nonconjugative ISG15

   Depositor

   Isidoro Martinez

   Article

   Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nonconjugative ISG15, LRAA

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MGAT1-GFP

   Plasmid

   #163647

   Purpose

   Expresses GFP tagged MGAT1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. EasyFusion T2A-H2B-GFP

   Plasmid

   #112851

   Purpose

   General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   No

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Sumihare Noji

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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