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pMuLE ENTR U6 L1-L4

Plasmid

#62112

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCLXSN-ING2

Plasmid

#13293

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-deltaNLS1

Plasmid

#22153

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

deletion of amino acids 297-304

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pBS Alpha 2 globin

Plasmid

#19231

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 globin (HBA1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pQTEV-NEUGRIN

Plasmid

#34804

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

NEUGRIN (NGRN Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3NT

Plasmid

#62259

Purpose

expression of A3NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR H1 L5-L2

Plasmid

#62103

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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myc-SpEAF pBacPAK8

Plasmid

#15320

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

Myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPRJi1

Plasmid

#59608

Purpose

Expression of shRNA against human PTPRJ

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPRJ (PTPRJ Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-ADAM21i2

Plasmid

#59605

Purpose

Expression of shRNA against human ADAM21

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ADAM21 (ADAM21 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pQTEV-C10orf83

Plasmid

#34827

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

C10orf83 (MORN4 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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PcDNA-DEST53-CypB-Del4 N-term GFP

Plasmid

#36137

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 42-216 of cyclophilin B

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSTTa

Plasmid

#248647

Purpose

E. coli protein expression vector encoding 2x N-terminal StrepII tags flanking an 11 residue flexible linker, thrombin and TEV protease sites, and an optional C-terminal FLAG tag.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Type

Empty backbone

Tags

2x StrepII, Thrombin site, TEV site and FLAG

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pME270

Plasmid

#238067

Purpose

Expresses ME-ABE ABE7.10-HRHSK with SpCas9 (NG)

Depositor

Alexis Komor

Insert

TadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include L36H, L51R, Y123H, C14…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pME464

Plasmid

#238068

Purpose

Expressed ME-ABE ABE7.10-HRHSK with SpRYCas9

Depositor

Alexis Komor

Insert

TadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include L36H, L51R, Y123H, C14…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-shSF3B1

Plasmid

#248800

Purpose

Knockdown of endogenous SF3B1 with shRNA targeting 3'UTR

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

shRNA targeting SF3B1 3'UTR

Use

Lentiviral

Promoter

hPGK

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HP17 sfGFP::P8

Plasmid

#235448

Purpose

Helper plasmid with sfGFP gene inserted at gene VIII locus

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-NatMX6

Plasmid

#240972

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; NatMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-EGFP

Plasmid

#246560

Purpose

Retroviral expression vector for EGFP

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

GFP

Use

Retroviral

Mutation

F64L, S65T (EGFP)

Promoter

MMLV LTR

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLP-PrhaBAD-pcepA-himar-chlorR

Plasmid

#238492

Purpose

Landing Pad with RhaRS/PrhaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

Depositor

Farren Isaacs

Article

Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

Type

Empty backbone

Expression

Bacterial

Promoter

RhaRS/PrhaBAD

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

Plasmid

#239237

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

Plasmid

#239240

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

Plasmid

#239242

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

Plasmid

#239258

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

Plasmid

#239259

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LSAM domain expression plasmid

Plasmid

#240215

Purpose

Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

Depositor

Elfriede Dall

Article

Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

Insert

LSAM (LGMN Human)

Tags

PC1pro

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-NanA-CSS-ET64

Plasmid

#234666

Purpose

Expression an ELP-tagged fusion enzyme of NanA and CSS

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

sialic acid aldolase
CMP-sialic acid synthetase

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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