We narrowed to 5,960 results for: actin

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

Plasmid

#196875

Purpose

Neuron-specific expression of LynGFP reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

Plasmid

#196896

Purpose

Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

N-Cadherin-2xHA (Cdh1 Mouse)

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-roxSTOProx-tRFP-pA-lox

Plasmid

#196888

Purpose

Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

turbo (t)RFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CAR

Plasmid

#171964

Purpose

Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

Plasmid

#171965

Purpose

Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AV13_pCAG.Cas9.gRNA.NT

Plasmid

#199260

Purpose

Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
Non-targeting (NT) control gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Myc/TSSK1

Plasmid

#199624

Purpose

Expression vector of mouse TSSK1. Myc-tag at N-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tssk1 mRNA (Tssk1 Mouse)

Tags

Myc-tag

Promoter

CAG

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

Plasmid

#186745

Purpose

dCas9-10XGcn4-P2A-mCherry expression construct

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

Cas9-10XGcn4-P2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex-Turbo/GFP

Plasmid

#197886

Purpose

Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

Turbo/GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEN-CAG-mRFP-PCNA pc2729

Plasmid

#166040

Purpose

Expresses mRFP tagged human PCNA in mammalian cells.

Depositor

Cristina Cardoso

Article

Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

Plasmid

#194249

Purpose

Expresses 3 non-targeting miRNAs

Depositor

Michael J Fox Foundation MJFF

Insert

miRNegative

Use

AAV and RNAi

Promoter

CAG

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG-C-Far

Plasmid

#193914

Purpose

Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Rep78-III/CAG/WPREΔU3

Plasmid

#190727

Purpose

Expression of AAV2 Rep78-III in mammalian cells

Depositor

Jakob Reiser

Article

Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

Insert

AAV2 Rep78-III

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP2

Plasmid

#178968

Purpose

Expresses miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

AAV

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CAG

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-RARa-VP16

Plasmid

#185559

Purpose

Ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Tags

VP16

Expression

Mammalian

Promoter

CAG

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-mNG-eDHFR(69K6)-Tiam1

Plasmid

#172102

Purpose

Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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