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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-N-22

Plasmid

#57420

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Expression

Yeast

Promoter

pFUS1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Scarlet-I

Plasmid

#193554

Purpose

Expresses mEGFP-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mScarlet-I

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pVG48

Plasmid

#164706

Purpose

rtTA under the control of PGK1 promoter

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

PGK1pr - rtTA - CYC1t

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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enz.079-GCN5

Plasmid

#154046

Purpose

Expresses GCN5 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone acetyltransferase GCN5 (GCN5 Budding Yeast)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2161_T2031E

Plasmid

#40395

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161, T2031E

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pAV0586

Plasmid

#133473

Purpose

Integration into fission yeast genome at lys3 locus with bsdMX selection cassette

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p413TEF-HST2

Plasmid

#51745

Purpose

overexpression of HST2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

HST2 (HST2 Budding Yeast)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2186_T2031E

Plasmid

#40389

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, T2031E

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus4x/cdc37/RocCOR LRRK2 1867-2176

Plasmid

#40398

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

WT aa1867-2176

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBACgus4x/cdc37/LRRK2 1867-2141

Plasmid

#40368

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

WT, aa1867-2141

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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YEplac181-GAL11

Plasmid

#8628

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

GAL11 (GAL11 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pAV0784

Plasmid

#133475

Purpose

Integration into fission yeast genome at his5 locus with bsdMX selection cassette

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0587

Plasmid

#133474

Purpose

Integration into fission yeast genome at his5 locus with bleMX selection cassette

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Type

Empty backbone

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBACgus4x/cdc37/RocCOR LRRK2 1867-2161

Plasmid

#40397

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

WT aa1867-2161

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pGEMHEZFCNGA5

Plasmid

#69861

Purpose

oocyte expression vector containing ZFCNGA5

Depositor

Gary Matthews

Article

Tetreault et al Biochem Biophys Res Commun. 2006 Sep 22;

Insert

ZFCNGA5 (cnga5 Zebrafish)

Use

Xenopus oocyte expression

Mutation

none

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only