170,823 results

Showing: 2251 - 2260 of 170823 results

Results per page:

Sort results by:

dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Article

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSS02 NAPstar4.3

Plasmid

#241222

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4.3 in Arabidopsis thaliana

Depositor

Markus Schwarzländer

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

cyt-NAPstar4.3

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-evoCAST-evoTnsAB

Plasmid

#234729

Purpose

Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

evoPseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubiquitin G76C

Plasmid

#86583

Purpose

Bacterial expression of Ubiquitin G76C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAVS1-SA-2A-NEO-CAG-RTTA3

Plasmid

#60431

Purpose

neomycin resistance; constitutive promoter driving reverse tet activator

Depositor

Paul Gadue

Article

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

rtTA3

Use

Zfn donor

Promoter

CAG

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag

Plasmid

#233217

Purpose

Mammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv [Preprint]. 2025 Feb 20:2023.10.

Insert

Anti-amyloid-β 40/42 nanobody (R3VQ)

Tags

Sortase tag, His tag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge