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pNB655

Plasmid

#60142

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
modRNAc0-p53DD

Plasmid

#176902

Purpose

IVT template plasmid for making p53DD modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

p53DD

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Multi-frame tag

Plasmid

#128610

Purpose

In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

MF tag

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgGOT1

Plasmid

#72874

Purpose

The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgGOT1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
HLA_B5101

Plasmid

#165011

Purpose

Expression of HLA-B*51:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-B*51:01 (HLA-B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-mKate2

Plasmid

#105941

Purpose

in vitro transcription, zf centrin N-terminally tagged with mkate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Centrin

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_HTC3xF_Puro_donor

Plasmid

#246392

Purpose

MTF2 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_KO_BSD_donor

Plasmid

#246396

Purpose

PHF1 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_BSD_donor

Plasmid

#246398

Purpose

PHF19 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_BSD_donor

Plasmid

#246400

Purpose

PHF19 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_PHF19_sgRNA

Plasmid

#246404

Purpose

Cas9/sgRNA expression plasmid targeting PHF19

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-TFIIB

Plasmid

#26675

Depositor

Sui Huang

Article

Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

Insert

TFIIB (GTF2B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 HA FoxO1

Plasmid

#14936

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pLKO-perox-tagRFP

Plasmid

#185600

Purpose

Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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