We narrowed to 6,471 results for: poly

1. pCMV6-AN-DDK_C344A_F354A RAD23A

   Plasmid

   #201579

   Purpose

   Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations C344A and F354A

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

   Plasmid

   #201547

   Purpose

   Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 276-339

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV6-AN-DDK_WT RAD23A

   Plasmid

   #201438

   Purpose

   Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19-TdTc253

   Plasmid

   #207464

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc253 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET19-TdTc188

   Plasmid

   #207463

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc188 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET19-TdTc180

   Plasmid

   #207462

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc180 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET19-TdTc302

   Plasmid

   #207461

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   TdTc302 (Dntt Mouse)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAP14

   Plasmid

   #186262

   Purpose

   wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   wgaAB bicistronic
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-2T-TRR-C421-G2304D

   Plasmid

   #182845

   Purpose

   mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   mutation G2304D (GGC/GAC)

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-2T-TRX-C751-E3616S

    Plasmid

    #182844

    Purpose

    mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRX (w Fly)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

    Plasmid

    #177145

    Purpose

    Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX4T3-GST-NQO1(Y128F)

    Plasmid

    #177141

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX4T3-GST-PolB(L301R/V303R/V306R)

    Plasmid

    #177137

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX4T3-GST-PolB(K206A/K244A/T304I)

    Plasmid

    #177134

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-DEST42-dre4

    Plasmid

    #170440

    Purpose

    Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    dre4 (dre4 Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-20

    Promoter

    T7

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-Flag-Rheb-12Rs

    Plasmid

    #165023

    Purpose

    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-12Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    All the lysine residues except K19 and K120 are c…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge