We narrowed to 18,890 results for: Tec

1. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

   Plasmid

   #203767

   Purpose

   For production of template RNA for use in PRINT, GFP under CBh promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Promoter

   CBh

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-HA/Net1A

   Plasmid

   #28262

   Depositor

   Jeffrey Frost

   Article

   Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

   Insert

   Net1A (Net1 Mouse)
   

   Tags

   2x HA

   Expression

   Mammalian

   Available Since

   April 11, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

   Plasmid

   #194217

   Purpose

   Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   UAS-ybTATA-tagBFP; PGK-mCitrine
   

   Use

   Lentiviral

   Promoter

   GAL4 UAS-ybTATA; PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TCARZ-SV40-GFP-PAmin-GF3-R4A22

   Plasmid

   #203769

   Purpose

   For production of template RNA for use in PRINT, GFP under SV40 promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Promoter

   SV40

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBT44c

   Plasmid

   #122600

   Purpose

   BE PACE selection, express C-intein-Cas9-ugi fusion

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   intein-dCas9-ugi fusion
   

   Use

   Synthetic Biology

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTPH418b

   Plasmid

   #185725

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTPH412

   Plasmid

   #185724

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-gp41-8N
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   ecTadA(8e) R26G

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-G1(4/5FTrp)RS

    Plasmid

    #207620

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pABM-D4-miR30-CypA

    Plasmid

    #132934

    Purpose

    Lentivector for CypA knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    CypA shRNA
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrEno

    Plasmid

    #115474

    Purpose

    Eno-promoter driven expression vector for protein production in Trichoderma reesei

    Depositor

    Steve Decker

    Article

    Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

    Insert

    Cel7A
    

    Use

    Unspecified

    Mutation

    Introns have been removed

    Promoter

    Trichoderma reesei Enolase (Eno) promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAM28

    Plasmid

    #234314

    Purpose

    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    U1367 Targeting RAM, Barcode 2
    

    Use

    Synthetic Biology

    Promoter

    P. CymRC

    Available Since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Promoter

    Consitutive lac

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BiP-mGFP

    Plasmid

    #62231

    Purpose

    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Tags

    mGFP

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-TaGu-ENT-3xF-ORF

    Plasmid

    #238488

    Purpose

    For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    R1109->A

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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