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L_NLS 27kDa

Plasmid

#201365

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP

Expression

Mammalian

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Diffusive 47kDa

Plasmid

#201336

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-3PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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RBM15B_3xRRM_pGEX

Plasmid

#135125

Purpose

Recombinant expression of RBM15B with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM15B (RBM15B Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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NOVA1_Full-length_pGEX

Plasmid

#135147

Purpose

Recombinant expression of NOVA1 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NOVA1 (NOVA1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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RBM6_RRM_pGEX

Plasmid

#135157

Purpose

Recombinant expression of RBM6 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM6 (RBM6 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Publication

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pANY2

Plasmid

#112196

Purpose

This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Publication

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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AXBEv2

Plasmid

#204606

Purpose

Mammalian expression of evolved adenine transversion base editor AXBEv2

Depositor

Dali Li

Publication

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM5-mSHMT2

Plasmid

#83905

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 2 (Shmt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL-C144A

Plasmid

#160285

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Publication

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TAOK2 (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rACh1h

Plasmid

#234412

Purpose

Expresses the red ACh sensor GRAB rACh1h in neurons

Depositor

Yulong Li

Publication

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1h

Use

AAV

Promoter

hSyn

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-GRAB_PGE2-1.0

Plasmid

#251355

Purpose

Express the PGE2 sensor GRAB_PGE2-1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wang et al Neuron. 2026 Jun 3;114(11):1922-1934.e5.

Insert

PGE2 sensor GRAB_PGE2-1.0

Use

AAV

Expression

Mammalian

Promoter

EF1α

Available since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pF-N-HiBiT-template

Plasmid

#200882

Purpose

template for N-terminal HiBIT-tagging

Depositor

Stefan Knapp

Publication

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HiBIT-linker

Expression

Mammalian

Mutation

WT

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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