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pGREAT17

Plasmid

#170905

Purpose

GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

p35SoTMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-CytERM-N-17

Plasmid

#56217

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CytERM

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pACUH-GFP1-10 sec

Plasmid

#172062

Purpose

UAS vector to express GFP1-10 in the ER lumen

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 sec

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGREAT4

Plasmid

#170892

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HyPerFLEX-Sec61b

Plasmid

#238940

Purpose

Mammalian expression of HyPerFLEX targeted to the cytosolic side of the endoplasmic reticulum membranes

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Sec61b

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pVTCL3

Plasmid

#234921

Purpose

All-in-one subcellar localization vector. Construct containing EGFP tag and cell endoplasmic reticulum-targeted mCherry for co-localization study in plants.

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Type

Empty backbone

Expression

Plant

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRER-PhoCl-Insert

Plasmid

#219675

Purpose

Caging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

RER-PhoCl

Tags

PhoCl

Expression

Mammalian

Promoter

TRE

Available Since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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AA416

Plasmid

#215992

Purpose

Fragmid fragment: (N' terminus) luminescent reporter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; V5_v1.1; NanoLuc_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBC113

Plasmid

#202275

Purpose

Level 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc140

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC115

Plasmid

#202277

Purpose

Level 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc142

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC108

Plasmid

#202247

Purpose

Level 2 for strain-specific barcode DNA tag with bc135 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc135

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC130

Plasmid

#202257

Purpose

Level 2 for strain-specific barcode DNA tag with bc157 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc157

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC131

Plasmid

#202258

Purpose

Level 2 for strain-specific barcode DNA tag with bc158 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc158

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC104

Plasmid

#202243

Purpose

Level 2 for strain-specific barcode DNA tag with bc131 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc131

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC105

Plasmid

#202244

Purpose

Level 2 for strain-specific barcode DNA tag with bc132 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc132

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC106

Plasmid

#202245

Purpose

Level 2 for strain-specific barcode DNA tag with bc133 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc133

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBC107

Plasmid

#202246

Purpose

Level 2 for strain-specific barcode DNA tag with bc134 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

Depositor

Paul Schulze-Lefert

Article

Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

Insert

bc134

Expression

Bacterial

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

Plasmid

#178910

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Mutation

CTG>AGT and TGC>CAT mir16+153 mutated

Promoter

SV40 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE16-mut1

Plasmid

#178906

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Mutation

CTG>AGT miR16-5p MRE1 mutated

Promoter

SV40 promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE153-mut4

Plasmid

#178909

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Mutation

TGC>CAT miR153-3p MRE mutated

Promoter

SV40 promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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