We narrowed to 9,400 results for: Coli
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pTpal-fdc
Plasmid#78288PurposeExpresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coliDepositorUseTagsExpressionBacterialMutation*see belowPromotertrcAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-aZEA
Plasmid#53286PurposeContains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coliDepositorInsertlcyE (LUT2 Mustard Weed)
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A2 (MACROH2A2 Human)
UseTagsmCeruleanExpressionBacterialMutationK167-K372PromoterAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56ā714; See paper for descriptionPromoterT7Available SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR1G108CP20X
Plasmid#85086PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
UseTagsExpressionBacterialMutationPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARH3-c002
Plasmid#173095PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS1222
Plasmid#63791PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection markerDepositorInserteGFP-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseGFP-Twin-Strep tagExpressionMutationPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating vā¦TagsExpressionMutationPromoterAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagExpressionMutationPromoterNoneAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSiMPla_N + pSiMPla_C
Plasmid#134314PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertampR_gp41-1 N-intein + ampR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETA
Plasmid#53316PurposeContains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.DepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlazZAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET-PLS-KP6_rpoA-U
Plasmid#190967PurposeTest the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
