We narrowed to 9,775 results for: Pol

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pCellFree_G03 PRKCA

Plasmid

#67115

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PRKCA Protein kinase C alpha type (PRKCA Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
CAG-mCherry-CrxshRNA

Plasmid

#73987

Purpose

Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Crx gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLMB684

Plasmid

#83779

Purpose

Xylose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

upstream region of RL2720 (rbsC)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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CRISPRmTmG2

Plasmid

#69992

Purpose

The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA that targets near LoxP sites

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-Chd1i4

Plasmid

#21908

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Chd1-i4

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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AL13Rb-HsfA2

Plasmid

#161012

Purpose

PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PPARG

Plasmid

#67099

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PPARG (PPARG Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLMB687

Plasmid

#83781

Purpose

Sucrose biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL120556 containing all of pRL120555

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMK110

Plasmid

#99535

Purpose

contains bgl operon sequence for E. coli in vitro transcription template

Depositor

Robert Landick

Article

Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

Insert

E. coli bgl antisense region containing bglG

Tags

None

Promoter

lambda Pr promoter used for in vitro transcriptio…

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046802

Plasmid

#145683

Purpose

Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKM149

Plasmid

#134178

Purpose

Bacterial expression of his-CENP-C 510-end

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

his-CENP-C 510-end (CENPC Human)

Expression

Bacterial

Mutation

aa 510-end

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pBM23-pyrF-NT

Plasmid

#174384

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-NT

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBM23-pyrF-CS

Plasmid

#174396

Purpose

pBM23 contained pyrF and mini-CRISPR.

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF-CS

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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FT1

Plasmid

#31435

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Fused Toes Homolog (AKTIP Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
CTAG_linker-eGFP

Plasmid

#136098

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_eGFP

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-mTurquoise2

Plasmid

#136100

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mTurquoise2

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS_GUS

Plasmid

#136084

Purpose

For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_GUS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_eGFP-linker

Plasmid

#136086

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_eGFP(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CDS12_mScarletI-linker

Plasmid

#136088

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mScarletI(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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