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GGE021

Plasmid

#120778

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2-3_Lip2

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE015

Plasmid

#120770

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Lip2

Use

Cloning/assembly plasmid

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Publication

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHS1376

Plasmid

#239829

Purpose

WT OrufIscB omegaRNA mammalian expression

Depositor

Feng Zhang

Publication

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB omegaRNA scaffold

Expression

Mammalian

Promoter

human U6

Available since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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BASIC_12_ATG-GFP-B0015

Plasmid

#68146

Purpose

storage vector for BASIC part: GFP(ORF)

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

ATG-GFP-B0015

Expression

Bacterial

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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BASIC_11_RBS34-GFP-B0015

Plasmid

#68145

Purpose

storage vector for BASIC part: RBS-GFP(ORF)

Depositor

Geoffrey Baldwin

Publication

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS-34-GFP-B0015

Expression

Bacterial

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p368_DG

Plasmid

#234691

Purpose

Golden gate domesticated pET28b with 3C-His6

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

3C-His6

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p345_DG

Plasmid

#234675

Purpose

Golden gate domesticated pET28b with His10-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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AA011

Plasmid

#215973

Purpose

Fragmid fragment: (N' terminus) for C>T base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA013

Plasmid

#215975

Purpose

Fragmid fragment: (N' terminus) for A>G base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P9-ehaA_LacI

Plasmid

#186317

Purpose

Plasmid for inducible E coli expression of the P9 helixCAM.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P9-ehaA

Expression

Bacterial

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Publication

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X4

Plasmid

#176154

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pWT009d

Plasmid

#107888

Purpose

R6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteria

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PBAD-EGFP

Expression

Bacterial

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pWT032a

Plasmid

#96879

Purpose

RFP reporter

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

mCherry

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK075

Plasmid

#65182

Purpose

Encodes LEU2 as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK053

Plasmid

#65160

Purpose

Encodes tADH1 as a Type 4 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tADH1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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