We narrowed to 10,383 results for: plasmids 101

1. pETM6-T7-VHH72

   Plasmid

   #202487

   Purpose

   Expresses the nanobody VHH72 with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VHH72
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLminP_Luc2P_RE49

   Plasmid

   #90388

   Purpose

   PPARg/RARa - PPARg gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   IRF3

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL

   Plasmid

   #218750

   Purpose

   Provides NOT intersectional (Flp NOT Cre) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1 hSynapsin1 NAPA-N SV40

   Plasmid

   #92282

   Purpose

   FRET probe; extracellular synapse targeted mCherry

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Neuronal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin1

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-LSL-mCherry-shPtbp1

   Plasmid

   #178591

   Purpose

   Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   miRE-Ptbp1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. APneg-SNAP25a

   Plasmid

   #168011

   Purpose

   negative selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   SNAP25-T7PAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC358

   Plasmid

   #122613

   Purpose

   TRE-delta-pes-10-GFP

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   7XTRE-delta-pes-10-GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB_PalkB_sfgfp_KanR

   Plasmid

   #166503

   Purpose

   Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PalkB

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEED-OLLAS:HA

    Plasmid

    #219647

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    OLLAS:HA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-hGFAP-mCherry-shPtbp1

    Plasmid

    #178589

    Purpose

    Expression of mCherry and a shRNA against Ptbp1 under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Ptbp1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP-SNAP25

    Plasmid

    #167995

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-hGFAP-mCherry-shLuci

    Plasmid

    #178588

    Purpose

    Expression of mCherry and a shRNA against luciferase under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP-VAMP4

    Plasmid

    #168000

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP4(I75-L91)-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZac2.1 hSynapsin1 FLEX NAPA-N SV40

    Plasmid

    #92283

    Purpose

    FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET26b_Nb.AT110

    Plasmid

    #135480

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CAG-LSL-mCherry-shLuci

    Plasmid

    #178590

    Purpose

    Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2_168

    Plasmid

    #199100

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge