We narrowed to 33,412 results for: IND

1. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiV_Neo_LKB1_CR

   Plasmid

   #108112

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change cytosine 333 to alanine (silent mutation)

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PatoM-OG241

   Plasmid

   #72842

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoM
   

   Expression

   Bacterial

   Promoter

   PatoM

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

   Plasmid

   #61940

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-156) GSGS (NFRKB Human)
   

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156, residues 97-100 (N…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

   Plasmid

   #61941

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-117) (NFRKB Human)
   

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 117, stop codon introdu…

   Promoter

   T7

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hsyn-ASAP5-Kv2.1-WPRE

   Plasmid

   #225707

   Purpose

   Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Has Service

   AAV1

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Promoter

   human synapsin

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212829

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Promoter

   CAG, TetOn

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

    Plasmid

    #175256

    Purpose

    SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-NIR-GECO2G

    Plasmid

    #159605

    Purpose

    AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    NIR-GECO2G
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-ASAP5-WPRE

    Plasmid

    #225709

    Purpose

    Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5
    

    Use

    AAV

    Promoter

    human synapsin

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pP{ELAV-GeneSwitch}

    Plasmid

    #83957

    Purpose

    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

    Depositor

    Haig Keshishian

    Article

    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

    Insert

    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)
    

    Expression

    Insect

    Promoter

    elav

    Available Since

    Dec. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-HIF1alpha P402A/P564A-pcDNA3

    Plasmid

    #18955

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-mito-R-GECO1

    Plasmid

    #46021

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Has Service

    AAV9

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Promoter

    EF1α

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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