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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-cAMP

Plasmid

#225250

Purpose

mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-cAMP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5pPBbsr2

Plasmid

#197351

Purpose

Encoding tFucci(CA)5.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

tFucci(CA)5

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Peredox

Plasmid

#73807

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

AAV

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-SUPREM

Plasmid

#205023

Purpose

Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

SUPer Rna Ecogii Methyltransferase

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-MCP-HALOnls

Plasmid

#121937

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

MCP-HALOnls

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-SPOTlight-U2GCR

Plasmid

#231889

Purpose

Cre-dependent reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Oliver et al eLife 2025 14:RP104457

Insert

DIO-SPOTlight-U2GCR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSAD-F3-NPEST-iCRE-2A-mCherryPEST

Plasmid

#99608

Purpose

Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

iCRE
mCherry

Use

Unspecified

Tags

PEST

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSKI015

Plasmid

#11570

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK8 HA (P#634)

Plasmid

#14649

Depositor

Matija Peterlin

Article

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk8 (CDK8 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Has Service

AAV8

Insert

pRSV

Use

AAV

Promoter

pRSV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Grx1-RoTq-On

Plasmid

#234467

Purpose

Mammalian expression of Grx1-RoTq-On

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

Grx1-RoTq-On

Use

AAV

Expression

Mammalian

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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RUBYm2

Plasmid

#232175

Purpose

Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

RUBY mutant2 and CaMV 35S promoter

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSLS.601

Plasmid

#184955

Purpose

Express -Eco1 ncRNA library variants

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 ncRNA (variants)

Expression

Bacterial

Mutation

Library members with mutations described in manus…

Promoter

T7/lac

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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