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pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

Plasmid

#73306

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

Q867E-Q939E-C935S-Q1011E-C1007S

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
mIFP-H1-10

Plasmid

#56222

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H1 (H1f0 Mouse)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Keratin-17

Plasmid

#56225

Purpose

Note- This plasmid contains Keratin 18. Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Keratin (KRT18 Human)

Tags

mIFP

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Clover-MAPTau-C-10

Plasmid

#56371

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MAPTau (MAPT Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MLM3733

Plasmid

#49964

Purpose

Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

3x Flag Tet1CD HB-6 (HBB Human)

Use

TALEN

Tags

3x FLAG

Promoter

EF1a

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
SL539

Plasmid

#49948

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only
JA1261

Plasmid

#49963

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD HB-6 (HBB Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
JA1290

Plasmid

#49968

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD HB-9 (HBB Human)

Use

TALEN

Promoter

EF1a

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
JA1252

Plasmid

#49956

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD HB-3 (HBB Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (G104R)

Plasmid

#29548

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G104R

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
AAV-tsC2ProT-mScarlet3-Cre

Plasmid

#248522

Purpose

This vector co-expresses Red Fluorescent Protein mScarlet3 and Cre Recombinase under the control of the truncated Tree Shrew Cerebellin 2 Promoter.

Depositor

Alev Erisir

Article

Kipcak et al Cell Rep Methods. 2026 Mar 23;6(3):10130

Insert

Truncated Tree Shrew Cerebellin 2 Promoter + mScarlet3 + Cre

Use

AAV

Expression

Mammalian

Promoter

Truncated Tree shrew Cerebellin 2 Promoter

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1(1-231)

Plasmid

#246498

Purpose

Recombinant protein expression of PCMTD1(1-231)

Depositor

Steven Clarke

Article

Warmack et al Biochemistry. 2022 May 17;61(10):879-894

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Deleted amino acids 232-357

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-714-1047-WT

Plasmid

#245307

Purpose

Expresses GAP domain of human p120RasGAP (residues 714-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-713

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET-p120RasGAP-581-1047-WT

Plasmid

#246487

Purpose

Expresses C2 and GAP domains of human p120RasGAP (residues 581-1047 wild type) in E. coli

Depositor

Titus Boggon

Article

Paul et al Methods. 2025 Dec;244:108-117. doi: 10.1

Insert

RASA1 (RASA1 Human)

Tags

6His

Expression

Bacterial

Mutation

deleted amino acids 1-580

Promoter

T7lac

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9P3

Plasmid

#210034

Purpose

Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro FLVCR1_sg5

Plasmid

#218522

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro CHKA_sg2

Plasmid

#218523

Purpose

sgRNA targeting human CHKA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v1 GFP FLVCR1_sg5

Plasmid

#218521

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CCM2_d410-444_eGFP

Plasmid

#208877

Purpose

Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids deletion : 410-444

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC-S230A

Plasmid

#200557

Purpose

Mammalian expression of secreted mouse CES2C S230A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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