We narrowed to 17,572 results for: bon;

1. pINSPECT_SigP:NLuc

   Plasmid

   #197263

   Purpose

   Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Expression

   Mammalian

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. W118-1_hZBTB7B_R363L

   Plasmid

   #180381

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R363L (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   R363L

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_hZBTB7B_D35N_R49Q

   Plasmid

   #180383

   Purpose

   lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_D35N/R49Q (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   D35N/R49Q

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. W118-1_hZBTB7B_R49H

   Plasmid

   #180382

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R49H (ZBTB7B Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   R49H

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/NDUFS2 _34-66/myc-His

    Plasmid

    #127495

    Purpose

    Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _34-66 (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _34-66

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/NDUFS2 E57A/myc-His

    Plasmid

    #127497

    Purpose

    Expresses myc-tagged NDUFS2/E57A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 E57A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 E57A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/NDUFS2 T58A/myc-His

    Plasmid

    #127498

    Purpose

    Expresses myc-tagged NDUFS2/T58A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 T58A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 T58A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/NDUFS2 H60A/myc-His

    Plasmid

    #127499

    Purpose

    Expresses myc-tagged NDUFS2/H60A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 H60A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 H60A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/NDUFS2 W61A/myc-His

    Plasmid

    #127500

    Purpose

    Expresses myc-tagged NDUFS2/W61A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 W61A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 W61A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/NDUFS2 K62A/myc-His

    Plasmid

    #127501

    Purpose

    Expresses myc-tagged NDUFS2/K62A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 K62A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 K62A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/NDUFS2 P63A/myc-His

    Plasmid

    #127502

    Purpose

    Expresses myc-tagged NDUFS2/P63A in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 P63A (NDUFS2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 P63A

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rescue myc-PCDHGC5 mRNA

    Plasmid

    #122229

    Purpose

    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Tags

    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

    Expression

    Mammalian

    Mutation

    Silent mutations are in five consecutive codons …

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLgw NKX2-5-V5-EcoDam

    Plasmid

    #98597

    Purpose

    Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR8/GW/TOPO-ELK1Y158A

    Plasmid

    #98628

    Purpose

    Gateway entry TOPO TA cloning vector ELK1 Y158A

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 Y158A (Elk1 Mouse)
    

    Use

    Topo ta cloning vector

    Mutation

    Y158A

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR8/GW/TOPO-ETS1

    Plasmid

    #98621

    Purpose

    Gateway entry TOPO TA cloning vector for ETS1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETS1 (Ets1 Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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