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Addgene

We narrowed to 10,599 results for: cat.1

Showing: 4561 - 4580 of 10599 results
  1. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid
    #208039
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
    Insert
    PML (isoform IVa; 3MAS) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pTRE3G-human cGASdeltaN R255E-HA

    Plasmid
    #186894
    Purpose
    Doxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation, R255E
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid
    #186899
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pTRE3G-FLAG-human cGASdeltaN-GFP

    Plasmid
    #186879
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal GFP fusion (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    FLAG and GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pTRE3G-human cGASdeltaN-OMM

    Plasmid
    #186881
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain (503-541 a.a.) fusion (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pTRE3G-human cGASdeltaN-Pex

    Plasmid
    #186882
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal PEX13 transmembrane domain (136-233 a.a.) fusion (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, PEX13 transmembrane domain…
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pTRE3G-human cGASdeltaN-ER

    Plasmid
    #186883
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal VAMP2 transmembrane domain (86-119 a.a.) fusion (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    N-terminal truncation, VAMP2 transmembrane domain…
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pLenti-CMV-human cGASdeltaN-OMM

    Plasmid
    #186887
    Purpose
    Expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain fusion (Adamts1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pTRE3G-human cGAS:156-522aa-HA

    Plasmid
    #186856
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (156-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pTRE3G-human cGAS:158-522aa-HA

    Plasmid
    #186857
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (158-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pTRE3G-human cGAS:162-522aa-HA

    Plasmid
    #186858
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (162-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pTRE3G-human cGAS:164-522aa-HA

    Plasmid
    #186859
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (164-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pTRE3G-human cGAS:166-522aa-HA

    Plasmid
    #186860
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (166-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pTRE3G-FLAG-human cGASdeltaN-HA

    Plasmid
    #186848
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4561 - 4580 of 10599 results