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pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Publication

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Expression

Mammalian

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_ mito_roGFP2-Orp1

Plasmid

#155042

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

mitochondrial targeting sequence and roGFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTriEx-mCherry-Zdk1-RhoA Q63L

Plasmid

#81058

Purpose

mammalian expression of Zdk1 tagged RhoA Q63L

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Zdk1
RhoA Q63L (RHOA Human)

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC33

Plasmid

#62330

Purpose

sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pwB3b

Plasmid

#32106

Depositor

Christopher Buck

Publication

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Inserts

BKV VP1
BKV VP3

Expression

Mammalian

Mutation

codon modified

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

Plasmid

#163064

Purpose

Entry vector for: GCaMP6F calcium sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMP6F

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

Plasmid

#218860

Purpose

10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Publication

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCG-ICAM(1235)-nrxn1b

Expression

Bacterial

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12.D11

Plasmid

#221151

Purpose

cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

Depositor

Urs Jenal

Publication

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Publication

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Publication

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A+B

Plasmid

#149370

Purpose

Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

Depositor

Tarek Abbas

Publication

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
sgRNA-A
sgRNA-B
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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NINJA TC

Plasmid

#159274

Purpose

Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

Depositor

Nikhil Joshi

Publication

Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

NINJA TC

Use

Mouse Targeting

Expression

Mammalian

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Publication

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICH50892

Plasmid

#48046

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pICH41800

Plasmid

#48020

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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