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Plasmid#162282PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only
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pEPQD0KN0024
Plasmid#162281PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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12.4kbVillin-ΔATG
Plasmid#19358DepositorInsert12.4kb Villin promoter
UseTagsExpressionBacterialMutationNo ATG - transgenes cloned downstream of the vill…PromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsaI
Plasmid#128649PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BsmBI
Plasmid#128648PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBJT002
Plasmid#225153PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTR235syn
Plasmid#133464PurposeConsensus sequence of Hop (Humulus lupulus) tandem repeats for cytogeneticsDepositorInsertHuluTR235
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits