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pGPI-SceI

Plasmid

#32060

Purpose

Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Type

Empty backbone

Use

Mutagenesis plasmid

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFPV25.1

Plasmid

#20668

Purpose

S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Tags

GFP mut3

Expression

Bacterial

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUMV19

Plasmid

#67161

Purpose

inducible operation of the mevalonate pathway in E. coli

Depositor

Tomohisa Kuzuyama

Article

Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

Insert

the mevalonate pathway genes

Expression

Bacterial

Promoter

lacZ

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-mEGFP-HDEL

Plasmid

#160467

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-mEGFP-HDEL

Expression

Yeast

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHR OVA/p2a/mCherry-CaaX

Plasmid

#113030

Purpose

for lentivirus production of ovalbumin and mcherry-caax

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

OVA/p2a/mCherry-CaaX

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHBFD1Bad

Plasmid

#53564

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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