1,878 results

1. pBN67

   Plasmid

   #86714

   Purpose

   DamID control plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

   Insert

   Phsp16.41::GFP::MYCDam
   

   Expression

   Worm

   Available Since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS544

   Plasmid

   #188882

   Purpose

   Integrating Pmex-5::Cas9 expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ1359 - Intron GG2 - smu-1

   Plasmid

   #159805

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - smu-1
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

   Plasmid

   #159861

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159867

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ889

   Plasmid

   #84826

   Purpose

   Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   smu-1 (smu-1 Nematode)
   

   Tags

   GFP

   Expression

   Bacterial and Worm

   Promoter

   Psmu-1

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-sCAG-GluClβ.CreON

   Plasmid

   #196996

   Purpose

   Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

   Insert

   GluClβ -YFP
   

   Use

   AAV and Cre/Lox

   Tags

   YFP

   Promoter

   short CAG

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA55

   Plasmid

   #59935

   Purpose

   gRNA for cleavage near sqt-1(e1350) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA2
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSM12

   Plasmid

   #139035

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   dendra2 (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGC95

    Plasmid

    #19611

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.41::FLP::let-858(3’)
    

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD291

    Plasmid

    #137019

    Purpose

    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    mKate2-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mKate2

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2215 - Intron GG3 - 900 bp

    Plasmid

    #159882

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG3 - 900 bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pmyo3::tag:sl1(G16C)

    Plasmid

    #79003

    Purpose

    Expression of exogenous SL1 in C. elegans muscle

    Depositor

    Xiao Liu, Ge Zhan

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G16C

    Promoter

    myo-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSiM3

    Plasmid

    #199382

    Purpose

    Encodes dual cistronic membrane localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    jGCaMP7s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Available Since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAP975-1

    Plasmid

    #99498

    Purpose

    TEV::linker::tagBFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::linker::tagBFP::3XFlag
    

    Expression

    Bacterial

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ907

    Plasmid

    #44473

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, [4-3] Gateway
    

    Use

    Gateway destination vector

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGC146

    Plasmid

    #19613

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Phsp-16.2::FLP::let-858(3’)-(Cbr)unc-119(+)
    

    Use

    Flp/frt

    Expression

    Worm

    Promoter

    hsp-16.2

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAWK61

    Plasmid

    #163642

    Purpose

    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
    

    Use

    CRISPR

    Tags

    2xmKate2 and GFP

    Expression

    Worm

    Promoter

    rps-27

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pID2.02

    Plasmid

    #26955

    Depositor

    Geraldine Seydoux

    Article

    Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

    Insert

    unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
    

    Expression

    Worm

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXF130

    Plasmid

    #139041

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mkate2 (includes STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJA59

    Plasmid

    #59932

    Purpose

    gRNA for cleavage at unc-109(n499) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-109(n499) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCM1.41

    Plasmid

    #21379

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    spe-11 promoter in pDONRP4P1R
    

    Use

    Gateway

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJW1592

    Plasmid

    #121059

    Purpose

    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    GFP^SEC^BioTag::AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

    Expression

    Worm

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJW1850

    Plasmid

    #154328

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pJW1849

    Plasmid

    #154327

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOX + kqt3

    Plasmid

    #16138

    Depositor

    Larry Salkoff

    Article

    Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

    Insert

    Caenorhabditis elegans potassium channel (kqt-3 Nematode)
    

    Use

    Xenopus oocyte expression

    Mutation

    lacks aa1-16 compared to GenBank reference AY5729…

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pAP758-1

    Plasmid

    #99494

    Purpose

    TEV::eGFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::3XFlag
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCM5.33

    Plasmid

    #19396

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fbf-1 3'utr in pDONRP2RP3
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

    Plasmid

    #159898

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
    

    Expression

    Worm

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge