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Plasmid#159681PurposeMammalian protein expression of HRAS-Halotag fusionDepositorInsertHalotag-Hs.HRAS (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)
Plasmid#208395PurposeTo generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationDB1delta/DB2deltaPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
Plasmid#134611PurposeMammalian expression of mouse KIF5A 378-1027DepositorInsertKIF5A (Kif5a Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M91-303: CMV51p> Halotag-Hs.NRAS
Plasmid#159682PurposeMammalian protein expression of NRAS-Halotag fusionDepositorInsertHalotag-Hs.NRAS (NRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
Plasmid#197066PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBPDepositorInsertEncapsulin
UseCRISPRTagsFKBP and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Halo-Tag-128-425-Akap9
Plasmid#196873PurposeExpression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from GolgiDepositorInsertHalo-Tag-Akap9 (128-425) (Akap9 Rat)
UseTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)
Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215001PurposeShuttling vector for a mitophagy reporterDepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai
Plasmid#202568PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai
Plasmid#202569PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
UseTagsHaloTagExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP)
Plasmid#215003PurposeShuttling vector for a Lysophagy reporterDepositorInsertLGALS3 (LGALS3 Human)
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)
Plasmid#215008PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST Halo-flag-CAD
Plasmid#188117PurposeExpresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tag and Halo tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
Plasmid#134612PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 375 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
UseTagsExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo K164A/H165A nsp1
Plasmid#175421PurposeExpresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHTN HaloTag CMV-neo/ERGIC2-variant
Plasmid#51477PurposeExpresses ERGIC2-variant in mammalian cells, HaloTag for cellular localizationDepositorInsertERGIC2 (ERGIC2 Human)
UseTagsHaloTagExpressionMammalianMutation4 base deletion at codon #189PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP
Plasmid#175522PurposeNuclear expression of HaloTag11 in mammalian cellsDepositorInsertH2B-HaloTag11
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E47: attB5r-Halotag-nostop-attB1r
Plasmid#162963PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHalotag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo delta 1-117 nsp1
Plasmid#175423PurposeExpresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 1-117 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationnsp1 C-terminusPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1
Plasmid#175814PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP
Plasmid#185686PurposeLow-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.DepositorInsertERmembrane-GST-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R124A/K125A nsp1
Plasmid#175417PurposeExpresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R124A/K125A nsp1
UseTags3XFLAG HaloExpressionMammalianMutationR124A and K125APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
R713-M50-303: CMV51p> Halotag-HRasHVR
Plasmid#159685PurposeMammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-HRasHVR (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M48-303: CMV51p> Halotag-Kras4aHVR
Plasmid#159684PurposeMammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-Kras4aHVR (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R703-M80-303: CMV51p> Halotag-Kras4bHVR
Plasmid#159683PurposeMammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-Kras4bHVR (KRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M52-303: CMV51p> Halotag-NRasHVR
Plasmid#159686PurposeMammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-NRasHVR (NRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only
Plasmid#69907PurposeExpresses a circular RNA containing the 5' half of GFP in mammalian cellsDepositorInsertGFP, ZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 C-terminal half
Plasmid#74603Purposeexpresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationamino acids 389-523 of LIC1PromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-STIM1 WPRE
Plasmid#236233PurposeAAV expression of mouse STIM1 internally tagged with HaloTagDepositorInsertHaloTag-Stim1 (Stim1 Mouse)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-Sec61b WPRE
Plasmid#236229PurposeAAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTagDepositorInsertHaloTag-Sec61b (SEC61B Human, Synthetic)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-STIM2 WPRE
Plasmid#236235PurposeAAV expression of human STIM2 internally tagged with HaloTagDepositorInsertHaloTag-STIM2 (STIM2 Human)
UseAAVTagsHaloTag in position 29ExpressionMutationSignal peptide from STIM1 and residues 15-746 of …Promoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-Jph3 WPRE
Plasmid#236238PurposeAAV expression of human Junctophilin3 N-terminally tagged with HaloTagDepositorInsertJunctophilin 3 (JPH3 Human)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11
Plasmid#209302PurposeExpression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTagDepositorInsertRC-I-1-H11
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-HaloTag-linker-SpyCatcher3
Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only