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We narrowed to 1,212 results for: ncl

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Showing: 441 - 460 of 1212 results

  1. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

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  7. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

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  8. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165566-plasmid-map-sequence-id-340094

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  9. pUAST-3x attB

    Plasmid
    #220329
    Purpose
    includes 3 standard UAS cassettes
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220329-plasmid-map-sequence-id-442491

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  10. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Promoter
    CMV immediate early
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101893-plasmid-map-sequence-id-211159

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  11. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84386-plasmid-map-sequence-id-272895

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  12. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid
    #86450
    Purpose
    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Tc'hsp5'3'UTR
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86450-plasmid-map-sequence-id-171108

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  13. pCI-SMN2-skipping-GLuc

    Plasmid
    #218670
    Purpose
    Alternative splicing signaling: signal decrease with Ex7 inclusion increasing
    Depositor
    Jingxin Wang
    Insert
    SMN2 (SMN2 )
    Expression
    Mammalian
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    218670-plasmid-map-sequence-id-450976

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  14. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Expression
    Bacterial
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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  15. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84388-plasmid-map-sequence-id-169692

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  16. pUPD2 MS2 (GB2665)

    Plasmid
    #160597
    Purpose
    CDS of MS2 protein with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    MS2
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160597-plasmid-map-sequence-id-322069

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  17. 2xUAS-1xhsp70-attB

    Plasmid
    #220317
    Purpose
    includes 2 UAS cassettes and 1 heat-shock inducible hsp70 casette
    Depositor
    Erdem Bangi
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    220317-plasmid-map-sequence-id-442487

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  18. pENTR CMVie-Lifeact-mCardinal L1-R5

    Plasmid
    #84392
    Purpose
    Multisite Gateway entry vector for expression of mCardinal-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mCardinal
    Expression
    Mammalian
    Promoter
    CMV immediate early
    Available Since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84392-plasmid-map-sequence-id-169693

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  19. pUPD2 dCas9 (GB2664)

    Plasmid
    #160596
    Purpose
    CDS of dCas9 with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160596-plasmid-map-sequence-id-322070

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  20. pICSL30069

    Plasmid
    #245571
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Twin Streptavidin-TurboID Fusion N-TAG (includes a 3' 3xGGGS Linker)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245571-plasmid-map-sequence-id-495565

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Showing: 441 - 460 of 1212 results