We narrowed to 1,183 results for: post

1. Venus2-Y705F-STAT3

   Plasmid

   #123173

   Purpose

   Expresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Y705F substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus2-S727A-STAT3

   Plasmid

   #123175

   Purpose

   Expresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   S727A substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTV001

   Plasmid

   #130265

   Purpose

   EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

   Insert

   eatA::His8
   

   Use

   Tags

   polyhistidine (internal)

   Expression

   Bacterial

   Mutation

   8His polyhistidine encoding region and XhoI site …

   Promoter

   araBAD

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HisGB1-Hif1a(776-826)_FIH

   Plasmid

   #99508

   Purpose

   Bacterial expression of hydroxylated Hif1a TAD (776-826)

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   FIH1 (HIF1AN Human)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Venus1-K49R-STAT3

   Plasmid

   #123166

   Purpose

   Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   K49R substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Venus2-K49R-STAT3

   Plasmid

   #123167

   Purpose

   Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   K49R substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Venus1-K140R-STAT3

   Plasmid

   #123168

   Purpose

   Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Use

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   K140R substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Venus2-K140R-STAT3

   Plasmid

   #123169

   Purpose

   Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   K140R substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus2-K685R-STAT3

   Plasmid

   #123171

   Purpose

   Expresses K695R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   K685R substitution

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

    Plasmid

    #233385

    Purpose

    PiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as marker

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGFR1(ecto+TM)-ADAR2dd
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-NEDD4L_ΔC2 (157)

    Plasmid

    #187720

    Purpose

    Express GST-tagged NEDD4L ΔC2 (157) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 157-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-156

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBacHTA-NEDD4L_FL

    Plasmid

    #187722

    Purpose

    Express His-tagged NEDD4L FL in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a(+)-NEDD4L_FL

    Plasmid

    #187705

    Purpose

    Express his-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX6P1-NEDD4L_FL

    Plasmid

    #187712

    Purpose

    Express GST-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1-NEDD4L_WW34

    Plasmid

    #187718

    Purpose

    Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-581 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    only residues 492-581

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-NEDD4L_HECT

    Plasmid

    #187719

    Purpose

    Express GST-tagged NEDD4L HECT domain in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 594-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-593

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pInducer-YAP1-S127A/S397A/S94A

    Plasmid

    #213586

    Purpose

    Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S127A, S397A, S94A

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge