We narrowed to 5,634 results for: pcas
-
Plasmid#62687PurposeMammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancerDepositorInsertamiR-eGFP419/amiR-eGFP123
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or49a-Gal4
Plasmid#63039PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or49a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or49a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Or63a-Gal4
Plasmid#63043PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or63a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or63a promoter
UseTagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectMutationPromoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
hs-leonardo
Plasmid#42065DepositorInsertleonardo (14-3-3zeta Fly)
UseTagsExpressionInsectMutationPromoterHsp70Available sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
spitz (9 kb)
Plasmid#38340DepositorInsertspitz (spi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e V106W)
Plasmid#138495PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-1
Plasmid#160701Purposederivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sitesDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionMutationLacZ bordered by BsaI restrction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PEmax-2A-BSD
Plasmid#234071PurposeFor lentiviral expression of PEmax with blasticidin resistanceDepositorInsertPEmax
UseCRISPR and LentiviralTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF-1α core promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXYB089
Plasmid#239628PurposePlant RNA Vision constructs with no gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01
stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB082
Plasmid#239578PurposePlant RNA Vision constructs with 41 bp gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01-gRNA01
gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB083
Plasmid#239579PurposePlant RNA Vision constructs with 82 bp gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01-gRNA03
gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only