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We narrowed to 9,938 results for: Rras

Showing: 9201 - 9240 of 9938 results

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pFUS2_a2b

Plasmid

#50682

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX Rad

Plasmid

#11227

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1995 Mar 3. 270(9):4805-12.

Insert

Rad (RRAD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N39: deletion of amino acids 1-39

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
MCHR1B_R (TAL 3009)

Plasmid

#36001

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting MCHR1B (mchr1b Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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MCHR1B_L (TAL 3008)

Plasmid

#36000

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting MCHR1B (mchr1b Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pBABE Rad C249

Plasmid

#11215

Depositor

Ronald Kahn

Article

Moyers et al J Biol Chem. 1997 May 2. 272(18):11832-9

Insert

Rad (RRAD Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C249: deletion of amino acids 248-308

Promoter

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
GDF2_R (TAL 3011)

Plasmid

#36003

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting GDF2 (gdf2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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GDF2_L (TAL 3010)

Plasmid

#36002

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting GDF2 (gdf2 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Olactb and hsp70

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.26_4xUAS_DSCP_GFP

Plasmid

#111931

Purpose

Vector to express GFP (under the control of the DSCP and an array of 4 UAS sites)

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

4xUAS GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJL50

Plasmid

#174068

Purpose

Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pTEF1-3NLS-97-3ZF-VP16

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-3

Plasmid

#164867

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-3

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Use

Tags

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2-Kre33

Plasmid

#99990

Purpose

Express yeast Kre33 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Use

Tags

Y2H activation domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Kre33

Plasmid

#99989

Purpose

Express yeast Kre33 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Use

Tags

Y2H binding domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pFUS2_a3a

Plasmid

#50683

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_a3b

Plasmid

#50684

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_a4a

Plasmid

#50685

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_a4b

Plasmid

#50686

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_b3

Plasmid

#50697

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS2_b2

Plasmid

#50696

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Use

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1053B

Plasmid

#184007

Purpose

Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAhh1 - U6c:sgRNAhh2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1053A

Plasmid

#184006

Purpose

Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNAeve1 - U6c:sgRNAeve2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pRhamBAD and ptet

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-mCherry-4A/4B-CAAX

Plasmid

#87699

Purpose

Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-4A/4B-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

Plasmid

#173230

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pFREE_Zeo

Plasmid

#92053

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Tags

Expression

Mutation

Promoter

pRhamBAD and ptet

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9201 - 9240 of 9938 results

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