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Nsp7-mCherry

Plasmid

#165134

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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Nsp13-mCherry

Plasmid

#165136

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-H6-TEV-cSrc

Plasmid

#214233

Purpose

Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

cSrc Kinase domain (SRC Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hCdt1-HA

Plasmid

#117500

Purpose

Expresses human Cdt1 fused to HA tag

Depositor

Domenico Maiorano

Article

Tsanov et al Nucleic Acids Res. 2014 Apr;42(6):3692-7

Insert

Cdc10-dependent transcript-1 (CDT1 1700 bp, Human)

Tags

HA

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp12

Plasmid

#165117

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp12 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV14-GINIP

Plasmid

#204845

Purpose

Express 3x FLAG tagged-GINIP in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pECE-M2-CDC27 wt

Plasmid

#31661

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog (CDC27 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-Nsp7

Plasmid

#165112

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KozATG-dL5-2XG4S-mCer3

Plasmid

#73207

Purpose

Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

Insert

KozATG-dL5-2XG4S-mCer3

Tags

The FAP and mCerulean3 are fused with 2 copies of…

Expression

Mammalian

Mutation

The dL5** FAP is E50D, L89S, also known as E52D/L…

Promoter

CMV

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBX_Sec61b_smGFP_Myc

Plasmid

#236092

Purpose

Constitutively encodes Sec61b-smGFP-Myc (sfGFP-based spaghetti monster fluorescent protein embedding Myc epitope tags), for targeting the endoplasmic reticulum.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Sec61b-smGFP-Myc (SEC61B Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGMKOSiE

Plasmid

#20865

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)

Expression

Mammalian

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP:FRB-FBAR

Plasmid

#223620

Purpose

Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

FBP17 (FNBP1 Human)

Tags

FKBP, TagBFP and mGFP, FRB

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_HECT

Plasmid

#187719

Purpose

Express GST-tagged NEDD4L HECT domain in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 594-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-593

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-BICRAL-FLAG-pgk-puro

Plasmid

#107732

Purpose

Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

Depositor

Emily Dykhuizen

Article

Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

Insert

BICRAL (BICRAL Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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