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pAc5.1B-lambdaN-HA-DmCup_418-1132_M

Plasmid

#146906

Purpose

Insect Expression of DmCup_418-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_418-1132 (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-L379AL383A_M

Plasmid

#146912

Purpose

Insect Expression of DmCup-L379AL383A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-L379AL383A (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-CS-DmCup_311-440_M

Plasmid

#146913

Purpose

Bacterial Expression of DmCup_311-440

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_311-440 (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos1_X

Plasmid

#147954

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-MAFB-sg

Plasmid

#194720

Purpose

px458 with guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

Cas9 (MAFB Streptococcus pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos2_X

Plasmid

#147960

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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3xTRE DREaMR 3p3DsR

Plasmid

#182206

Purpose

3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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1xTRE DREaMR 3p3DsR

Plasmid

#182204

Purpose

1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_771-1132_C

Plasmid

#146030

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_771-1132_C

Plasmid

#146034

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

Plasmid

#146035

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-Y342AL347A (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_28_0570_WT

Plasmid

#171642

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.28.0570

Tags

Myc-His

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_31_2000_WT

Plasmid

#171643

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.31.2000

Tags

Myc-His

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PKM-Halo

Plasmid

#175321

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PKM (PKM Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTN_Halo-STMN1

Plasmid

#175299

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

STMN1 (STMN1 Human)

Use

Cell free expression

Tags

6xHis-HaloTag

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD30

Plasmid

#173033

Purpose

pQE30 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

S. flexneri 2a wzzBSF gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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prrnBT1_STD

Plasmid

#165803

Purpose

Bacterial rrnB gene T1 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT1

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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prrnBT2_STD

Plasmid

#165804

Purpose

Bacterial rrnB gene T2 terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

rrnBT2

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pHPH

Plasmid

#165839

Purpose

hygromycin B resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

hph

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBLE

Plasmid

#165840

Purpose

Zeocin/Phleomycin resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

ble

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pWhite_CDS

Plasmid

#165846

Purpose

White gene CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Dm White CDS

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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psfGFP

Plasmid

#165829

Purpose

sfGFP green fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

sfGFP

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNPTII

Plasmid

#165836

Purpose

G418 sulfate (geneticin) resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

nptII

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPAC

Plasmid

#165837

Purpose

puromycin resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

pac

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pL3S2P21_STD

Plasmid

#165805

Purpose

Synthetic bacterial terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

L3S2P21

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pspy_STD

Plasmid

#165806

Purpose

bacterial terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

spy

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD111

Plasmid

#172957

Purpose

pRMCD77 encoding Wzz mutant G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G311A mutant gene

Expression

Bacterial

Mutation

D168G and N33D- please see depositor comment

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBL-2

Plasmid

#162769

Purpose

Minimal backbone with AmpR

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Synthetic Biology; Molecular cloning

Promoter

no

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pG-02

Plasmid

#166933

Purpose

Target AD1.A5 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S5 +RBS - Block G- Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-03

Plasmid

#166927

Purpose

STAR-AD1.A6 part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star Antisense AD1.A6 - Block C - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-02

Plasmid

#166926

Purpose

STAR-AD1.A5 part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star Antisense AD1.A5- Block C- Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Article

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHC_DYOLacI-R

Plasmid

#102922

Purpose

pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

sfGFP

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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LI A-B phrpB

Plasmid

#104514

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

hrpB promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI A-B pEPS

Plasmid

#104515

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

eps promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LI A-B pGala7

Plasmid

#104516

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gala7 promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LI C-D Dest

Plasmid

#104518

Purpose

LI Gateway cassette

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gateway cassette

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI D-E V5

Plasmid

#104519

Purpose

LI C-terminal module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

3*V5 gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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