We narrowed to 7,466 results for: addgene/1000

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pET32a-hEPGN

Plasmid

#199232

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epithelial mitogen isoform 6 (EPGN Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-ATG9A-N

Plasmid

#198530

Purpose

Expression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP-L

Plasmid

#198528

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP-L (FHIP1A Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH

Plasmid

#148878

Purpose

Mammalian Expression of TNFalpha-ARE-polyA-Malat

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

TNFalpha-ARE-polyA-Malat (TNF Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_571-676-GB1_AG

Plasmid

#148745

Purpose

Bacterial Expression of HseIF4G_571-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_571-676 (EIF4G1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194367

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194368

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194369

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194361

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

Plasmid

#148519

Purpose

Mammalian Expression of HseIF4G1_615-654-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-L640AV646A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-D830A/E831A

Plasmid

#188085

Purpose

Expresses human ATG9-D830A/E831A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

D830A/E831A

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (801-839)

Plasmid

#188089

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (723-839)

Plasmid

#188088

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (723-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9 (801-839)

Plasmid

#188093

Purpose

To make monomeric ATG9 C-terminal tail for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (801-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (828-839)

Plasmid

#188090

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (828-839)

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-HseIF4E_AF

Plasmid

#148669

Purpose

Bacterial Expression of HseIF4E

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E (EIF4E Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence of i…

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-α-tubulin

Plasmid

#136840

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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