We narrowed to 5,906 results for: pcDNA 3.1

1. optGluCl beta Y182F ECFP

   Plasmid

   #15105

   Depositor

   Henry Lester

   Article

   Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

   Insert

   GluCl beta (glc-2 Nematode)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA, Y182F

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus rGBD

   Plasmid

   #99015

   Purpose

   Used as a RhoA-GTP sensor

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   rGBD (Rtkn Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4xHRE_minSV40-sfGFP-CMV_dsRed

   Plasmid

   #117400

   Purpose

   sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-CD28TMD-NTEVp(WT)

   Plasmid

   #137826

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(WT)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmyc-GFP-TNRC6A-NES-mut

   Plasmid

   #42001

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   F951A, F955A, I958A, F960A

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNLRP11major.MYC

   Plasmid

   #131196

   Purpose

   Mammalian expression of human NLRP11 188S

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   NLRP11 188S (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   188S

   Promoter

   pCMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFRB-myc

   Plasmid

   #20226

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. eTC GFP ß-actin ΔZip

   Plasmid

   #27124

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   This construct lacks β-actin 3′UTR and therefore …

   Promoter

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-YL1

    Plasmid

    #15333

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

    Insert

    transcription factor-like 1 (VPS72 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

11. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRPML1-MYC

    Plasmid

    #18824

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. OSCA1.2-PP-GFP

    Plasmid

    #135390

    Purpose

    Expresses OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    EGFP tag connected to the gene via a precision pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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