We narrowed to 165,307 results for: addgene

1. dSaCas9-VPR

   Plasmid

   #205251

   Purpose

   In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSaCas9-VPR
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-spCas9-SB

   Plasmid

   #169919

   Purpose

   Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-SB100X
   

   Use

   CRISPR

   Promoter

   CAG

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-EN1201

   Plasmid

   #92144

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
   

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPXL-Sox9

   Plasmid

   #36979

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVtight-RFP-IRES-CD

   Plasmid

   #126678

   Purpose

   Doxycycline inducible expression of CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   RFP-IRES-CD (FCY1 Budding Yeast)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR v2-sgControl

   Plasmid

   #230080

   Purpose

   Crispr knock out control

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   non-specific sequence control
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Marathon-RT pentamutant for Split-PE (pEH107)

   Plasmid

   #190110

   Purpose

   Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, N26R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_PEmax-2A-BSD

    Plasmid

    #234071

    Purpose

    For lentiviral expression of PEmax with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. XRE-H2B-eGFP

    Plasmid

    #182294

    Purpose

    Fluorescent reporter for AhR activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    Xenobiotic Response Element (CYP1A1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    XRE

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro HA PIK3CB

    Plasmid

    #12526

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CB (PIK3CB Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-CAG-flex-GFP-4x6T

    Plasmid

    #196418

    Purpose

    Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p27-IRES-mCherry

    Plasmid

    #80143

    Purpose

    retroviral expression of p27 and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    p27 (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158990

    Purpose

    Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol1-UAS:GtACR1-tdTomato

    Plasmid

    #124235

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    GtACR1-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Promoter

    14xUAS-E1b

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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