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pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1

Plasmid

#22027

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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a1Ha-pcDNA3

Plasmid

#45809

Depositor

Edward Perez-Reyes

Article

Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Insert

a1Ha (CACNA1H Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBB542

Plasmid

#27395

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, groESL

Expression

Bacterial

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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CaVα2δ1

Plasmid

#26575

Depositor

Diane Lipscombe

Article

Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

Insert

Cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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