We narrowed to 28,406 results for: REP

1. pJK643

   Plasmid

   #71628

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr mutant (TdPurE2-S38T)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to threonine

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK644

   Plasmid

   #71629

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp mutant (TdPurE2-S38D)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to aspartate

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC8-SZ

   Plasmid

   #17330

   Depositor

   Takeshi Sano

   Article

   Argarana et al Nucleic Acids Res. 1986 Feb 25. 14(4):18

   Insert

   streptavidin
   

   Use

   For maintenance

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pJK692

   Plasmid

   #71643

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His17>Trp/Ser38>Thr mutant (TdPurE2-H17W,S38T)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-17 to tryptophan, serine-38 to …

   Promoter

   T7

   Available since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK653

   Plasmid

   #71630

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Thr/Phe79>Trp mutant (TdPurE2-S38T,F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to threonine, phenylalanine-79 …

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK654

   Plasmid

   #71631

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to alanine, phenylalanine-79 to…

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK655

   Plasmid

   #71632

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asp/Phe79>Trp mutant (TdPurE2-S38D,F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to aspartate, phenylalanine-79 …

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK656

   Plasmid

   #71633

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to valine, phenylalanine-79 to …

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK659

   Plasmid

   #71634

   Purpose

   Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes serine-38 to cysteine, phenylalanine-79 t…

   Promoter

   T7

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK660

    Plasmid

    #71635

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to methionine, phenylalanine-79…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK661

    Plasmid

    #71636

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-41 to arginine, phenylalanine-79 t…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK662

    Plasmid

    #71637

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes phenylalanine-79 to tryptophan, cysteine-…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK689

    Plasmid

    #71642

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes serine-38 to asparagine, phenylalanine-79…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK697

    Plasmid

    #71646

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-15 to tryptophan, serine-38 to…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196078

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196076

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196082

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196080

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196074

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK528

    Plasmid

    #71625

    Purpose

    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole carboxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes methionine-105 to serine, isoleucine-109 …

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIB165

    Plasmid

    #90188

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21a-Traptavidin

    Plasmid

    #24753

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 Apr 11. ():.

    Insert

    Traptavidin
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G an…

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIB190

    Plasmid

    #90197

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIB167

    Plasmid

    #90190

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIB169

    Plasmid

    #90192

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIB163

    Plasmid

    #90186

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIAGO-amphDI

    Plasmid

    #114459

    Purpose

    Expression of AmphDI glycosyltansferase in streptomycetes

    Depositor

    Patrick Caffrey

    Article

    Stephens et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    amphDI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermE

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge