Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 52,941 results for: plasmid

Showing: 11651 - 11700 of 52941 results

  1. pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

    Plasmid
    #146006
    Purpose
    Insect Expression of DmSmaug-del584-762
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug-del584-762 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    7 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146006-plasmid-map-sequence-id-288451

    Enlarge

  2. pAc5.1B-lambdaN-HA-DmSmaug_584-999-V5His6_C

    Plasmid
    #146012
    Purpose
    Insect Expression of DmSmaug_584-999
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug_584-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    5 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146012-plasmid-map-sequence-id-288457

    Enlarge

  3. pAc5.1B-lambdaN-HA-DmSmaug-A642H-V5His6_C

    Plasmid
    #146014
    Purpose
    Insect Expression of DmSmaug-A642H
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug-A642H (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and a deletion of the AA 961-…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146014-plasmid-map-sequence-id-288459

    Enlarge

  5. pAc5.1C-Fluc-nanos_C

    Plasmid
    #146018
    Purpose
    Insect Expression of nanos-3UTR
    Depositor
    Elisa Izaurralde
    Insert
    nanos-3UTR (nos Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    3'UTR contains deletions and mutations compa…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146018-plasmid-map-sequence-id-288463

    Enlarge

  8. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-B*008:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180455-plasmid-map-sequence-id-355081

    Enlarge

  9. pTCF194 Mamu-E*02:04

    Plasmid
    #180461
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    Mamu-E*02:04
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180461-plasmid-map-sequence-id-355080

    Enlarge

  10. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid
    #178046
    Purpose
    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Renilla_713 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178046-plasmid-map-sequence-id-352920

    Enlarge

  11. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid
    #178045
    Purpose
    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Luciferase_1309 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178045-plasmid-map-sequence-id-352919

    Enlarge

  12. p3xFLAG-CMV-fly Dicer

    Plasmid
    #171559
    Purpose
    Encodes fly Dicer protein
    Depositor
    Yan Zeng
    Insert
    Dcr-1(CG4792) (Dcr-1 Fly)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171559-plasmid-map-sequence-id-337965

    Enlarge

  13. pKP2032

    Plasmid
    #167651
    Purpose
    Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Depositor
    Kathleen Postle
    Insert
    his6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167651-plasmid-map-sequence-id-337296

    Enlarge

  14. pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

    Plasmid
    #146013
    Purpose
    Insect Expression of DmSmaug_763-999
    Depositor
    Elisa Izaurralde
    Insert
    DmSmaug_763-999 (smg Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations and a deletion of the AA 961-9…
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    146013-plasmid-map-sequence-id-333962

    Enlarge

  15. pcDNA3-3NFLAG-mLIG3-179-936

    Plasmid
    #159316
    Purpose
    Mammalian cell expression of mouse LIG3-179-936
    Depositor
    Jianjun Zhao
    Insert
    mLIG3-179-936 (Lig3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159316-plasmid-map-sequence-id-311856

    Enlarge

  16. WDR55

    Plasmid
    #167844
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR55 (WDR55 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 1, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167844-plasmid-map-sequence-id-329228

    Enlarge

  17. 6WAT

    Plasmid
    #162268
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF1 (PHF1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162268-plasmid-map-sequence-id-319831

    Enlarge

  18. 6PED

    Plasmid
    #162265
    Purpose
    Baculovirus expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    HEMK2 (N6AMT1 Human)
    Use
    Tags
    6xHis-Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162265-plasmid-map-sequence-id-319828

    Enlarge

  19. 6VYC

    Plasmid
    #162266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR91 (WDR91 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162266-plasmid-map-sequence-id-319825

    Enlarge

  20. pKLV-gRNA-BTRC-MGMT_2

    Plasmid
    #136416
    Purpose
    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136416-plasmid-map-sequence-id-271757

    Enlarge

  21. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133579-plasmid-map-sequence-id-274429

    Enlarge

  22. pENTR-Hu_TTK1(WT)

    Plasmid
    #136354
    Purpose
    Human TTK (Mps1) cDNA in Gateway pENTR
    Depositor
    Guillermo de Cárcer
    Insert
    TTK (TTK Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136354-plasmid-map-sequence-id-270746

    Enlarge

  23. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

    Enlarge

  24. pcDNA-FLAG-Intu

    Plasmid
    #128869
    Purpose
    For mammalian expression of FLAG-tagged mouse Inturned
    Depositor
    Aimin Liu
    Insert
    Inturned (Intu Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    128869-plasmid-map-sequence-id-252120

    Enlarge

  25. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

    Enlarge

  26. Myc var1 K397R

    Plasmid
    #127285
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K397R by Q5 mutagenesis
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127285-plasmid-map-sequence-id-249995

    Enlarge

  27. Myc var1

    Plasmid
    #127284
    Purpose
    Expresses Myc to see if degraded by ELLs
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    transcript variant 1
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127284-plasmid-map-sequence-id-249994

    Enlarge

  32. pLV-EF1a-V5-Ccdc167

    Plasmid
    #120242
    Purpose
    Expresses V5-tagged Ccdc167 in lentiviral vector
    Depositor
    Larry Gerace
    Insert
    Ccdc167 (Ccdc167 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120242-plasmid-map-sequence-id-232814

    Enlarge

  33. 6CW0 (cgd2_2690)

    Plasmid
    #119965
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_2690
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1-105
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119965-plasmid-map-sequence-id-232689

    Enlarge

  34. pCAG-Scarlet-Borcs7-Q87X

    Plasmid
    #118751
    Purpose
    Expresses scarlet tagged Borcs7-Q87X
    Depositor
    Beverly Koller
    Insert
    Borcs7-Q87X cDNA (2010012O05Rik Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Q87X
    Promoter
    CAG
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118751-plasmid-map-sequence-id-229085

    Enlarge

  35. BK beta4 ECFP

    Plasmid
    #113564
    Purpose
    human BK beta4 subunit in C-term translational frame with ECFP (ECFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb4 (KCNMB4 Human)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113564-plasmid-map-sequence-id-218936

    Enlarge

  36. C2 H5 png-Ha/His in Casper

    Plasmid
    #112969
    Purpose
    P element vector for transposon insertion of png-HA/His into Drosophila genome
    Depositor
    Terry Orr-Weaver
    Insert
    png-HA/His (png Fly)
    Use
    P insertion vector
    Tags
    HA/His epitope
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112969-plasmid-map-sequence-id-217662

    Enlarge

  37. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

    Enlarge

  38. hC73M-HA

    Plasmid
    #111528
    Purpose
    Expresses human KCNB1 C73M in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73M-Kv2.1 + HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73M
    Promoter
    CMV
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    111528-plasmid-map-sequence-id-213350

    Enlarge

  39. hC73L-HA

    Plasmid
    #111527
    Purpose
    Expresses human KCNB1 C73L in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73L-Kv2.1-HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73L
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111527-plasmid-map-sequence-id-211650

    Enlarge

  40. pUPD2b:TMV-3NTR

    Plasmid
    #106218
    Purpose
    Provides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)
    Depositor
    Tomáš Moravec
    Insert
    Infectious clone of Tobacco mosaic virus, 3' part
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106218-plasmid-map-sequence-id-208888

    Enlarge

  41. pT7-7 asyn S129W

    Plasmid
    #105744
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein S129W (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    S129W
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105744-plasmid-map-sequence-id-207297

    Enlarge

  42. pT7-7 asyn K32R

    Plasmid
    #105739
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein K32R (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K32R
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105739-plasmid-map-sequence-id-207290

    Enlarge

  43. pET11a asyn Q79N (TAC-->TAT)

    Plasmid
    #105733
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Q79N
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105733-plasmid-map-sequence-id-207258

    Enlarge

  44. HA-mus-Bnip3_Exon(2+3)

    Plasmid
    #100794
    Purpose
    Mammalian expression of Bnip3_Exon2+3 splice variant
    Depositor
    Joseph Gordon
    Insert
    mouse-BNIP3_Exon(2+3) (Bnip3 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Exon2 and Exon3 deletion
    Promoter
    CMV
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100794-plasmid-map-sequence-id-193240

    Enlarge

  45. pAM-35s-Snrk2.3-YFPn-1

    Plasmid
    #102391
    Purpose
    split YFP. Plant expression of Snrk2.3-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    SNRK2.3 (SNRK2.3 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102391-plasmid-map-sequence-id-196616

    Enlarge

  46. CMYA5tnC/pBSSK+

    Plasmid
    #98119
    Purpose
    CMYA5 (NM_153610, nt 11648 to 12279) in pBSSK+ (minimum binding region to titin)
    Depositor
    Rita Shiang
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98119-plasmid-map-sequence-id-191012

    Enlarge

  47. pGAD424-hL1

    Plasmid
    #89423
    Purpose
    Two-hybrid system
    Depositor
    Fritz Rathjen
    Insert
    L1CAM (L1CAM Human)
    Use
    Tags
    Gal4 AD
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89423-plasmid-map-sequence-id-177566

    Enlarge

  50. pAS2-mCALEBcyt (aa 465-539)

    Plasmid
    #89408
    Purpose
    Two-hybrid system
    Depositor
    Fritz Rathjen
    Insert
    CSPG5 (Cspg5 Mouse)
    Use
    Tags
    GAL4 DBD
    Expression
    Yeast
    Mutation
    aa 465-539
    Promoter
    ADH1
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89408-plasmid-map-sequence-id-177484

    Enlarge

Showing: 11651 - 11700 of 52941 results