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pAJ50

Plasmid

#14918

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

enhanced monomeric red fluorescent protein

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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a5-ecto-pcDNA31hygro

Plasmid

#113386

Purpose

Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human alpha 5 ectodomain

Tags

Strep II tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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IRE-miR21-SunTag-TREAT

Plasmid

#196932

Purpose

SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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RhoQ

Plasmid

#55695

Purpose

expression of mouse RhoQ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoQ (Rhoq Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 S100A1

Plasmid

#67048

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

S100A1 (S100A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Tags

GFP

Expression

Yeast

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Tags

GFP

Expression

Yeast

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B-V5

Plasmid

#35931

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBacPAK p110b WT

Plasmid

#55724

Purpose

His3 GST tagged WT p110 beta

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 beta

Tags

His3 GST

Promoter

polyhedrin promoter

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
TOPO mKO2

Plasmid

#68442

Purpose

TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKusabira-Orange2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670

Plasmid

#82621

Purpose

Expresses NLS-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB1_omega1R

Plasmid

#68236

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_D5802

Plasmid

#176880

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10:Luc+:RbcsE9

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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RAP2B

Plasmid

#55667

Purpose

expression of mouse RAP2B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2B (Rap2b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
Wnt7B STOP

Plasmid

#35878

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pET-11a (GB1 WT - A34F)

Plasmid

#136609

Purpose

Expresses GB1 WT mutant (A34F)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT mutant (A34F)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SREBF2

Plasmid

#67129

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SREBF2 (SREBF2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only