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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-P2A-GFP no-pA

Plasmid

#80826

Purpose

P2A followed by GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-GFP no-pA

Expression

Mammalian

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2_163

Plasmid

#199095

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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UAS::TVA

Plasmid

#164487

Purpose

Expression of TVA

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Insert

TVA (CD320 Chicken)

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFUM

Plasmid

#133130

Purpose

A recombinant plasmid expressing fum (fumarase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

fumarase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti pTERT sgRNA1(MS2) mCherry

Plasmid

#171054

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA1(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D3

Plasmid

#10912

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D3 (CCND3 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCfB8787

Plasmid

#126921

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-AsCas12j-2

Plasmid

#191655

Purpose

All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

Depositor

Fong Tian Wong

Article

Tan et al bioRxiv 2021.10.28.465406

Insert

AsCas12j-2

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB8788

Plasmid

#126922

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pJDC124

Plasmid

#157877

Purpose

mCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

mCherry

Expression

Bacterial

Promoter

phsp60

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8748

Plasmid

#126918

Purpose

Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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