We narrowed to 18,470 results for: Met

1. mIFP24-LaminA-C-18

   Plasmid

   #56267

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Tags

   mIFP24

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. AAV-CAG-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #105834

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LMP AMPK alpha2 hp1

   Plasmid

   #89490

   Purpose

   shRNA against AMPK alpha2 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   hairpin against mouse/human AMPKalpha2 (PRKAA2, Prkaa2 )
   

   Use

   Mouse Targeting and Retroviral

   Expression

   Mammalian

   Promoter

   LMP

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

4. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129028

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mIFP-LaminA-C-18

   Plasmid

   #56226

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Tags

   mIFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. FLINC-AKAR1(TA)-CAAX

   Plasmid

   #87706

   Purpose

   Negative-control mutant of FLINC-AKAR1-CAAX.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   FLINC-AKAR1(TA)-CAAX
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKAsub-TagRFPT-CAAX

   Plasmid

   #87712

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   PKAsub-TagRFPT-CAAX
   

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

   Plasmid

   #47905

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mIFP12-Farnesyl-5

    Plasmid

    #56251

    Purpose

    Localization: Plasma Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mIFP12-LaminA-C-18

    Plasmid

    #56256

    Purpose

    Localization: Nuclear Envelope, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    LaminA (LMNA Human)
    

    Tags

    mIFP12

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Mutation

    H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JA412

    Plasmid

    #49935

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD KL-1 (KLF4 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SL357

    Plasmid

    #49936

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1FL KL-1 (KLF4 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129036

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (huTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEAHISMRSA2676

    Plasmid

    #96970

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 2676

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Sar2676
    

    Tags

    N-ter TEV protease cleavable 6His

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEAHISMRSAMVAK

    Plasmid

    #97000

    Purpose

    expression of methicillin-resistant Staphylococcus aureus MVAK protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Mevalonate kinase
    

    Tags

    N-ter TEV protease cleavable 6His

    Mutation

    none

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge