We narrowed to 6,944 results for: SIM;
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Plasmid#123931PurposeEncodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPhic31attB tagBFP destination vector
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_026
Plasmid#123906PurposeEncodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInserthuman non targeting sgRNA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_028
Plasmid#123908PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA2 -hThal5.10-2_hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_029
Plasmid#123909PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA3 -hThal5.10-2_hg18
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_013
Plasmid#123867PurposeEncodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK systemDepositorInsertWPRE-Blast (no terminator)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_018
Plasmid#123733PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertspdCas9(1-204)-frb-D10A-ERT2
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG_Ptet*_ToeRep_N01_switch
Plasmid#132737PurposeInducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_ToeRep_N01_switch
ExpressionBacterialAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND3_L11_S11_S13_S19
Plasmid#132731PurposeT7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19DepositorInsert3WJ_NAND3_L11_S11_S13_S19
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-Citrine-SV40pA-FKF
Plasmid#119865PurposeDonor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Tags2x myristoylationExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m4
Plasmid#84723PurposepVR2 but with G to U and A to C mutations following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutation at the first nucleotide after the…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m5
Plasmid#84724PurposepVR2 but with an insertion of 1 U immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationinsertion of 1 U between positions 39 and 40 of a…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m6
Plasmid#84725PurposepVR2 but with an insertion of 2 U's immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationinsertion of 2 Us between positions 39 and 40 of …Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m7
Plasmid#84726PurposepVR2 but with a G to U mutation immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutation at position 41 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m8
Plasmid#84727PurposepVR2 but with two G to U mutations immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationG to U mutations at positions 40 and 41 of annota…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR
Plasmid#84718PurposeE. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilisDepositorInsertsE. coli RecA promoter
5' UTR of queC gene
ExpressionBacterialAvailable SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m1
Plasmid#84720PurposepVR2 but with a C to U mutation in the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutationC to U mutation at position 13 of annotated "…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB367
Plasmid#110277PurposegHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHEEE_02
Tags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialPromoterT7Available SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-DXMT
Plasmid#79822PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantPromoterCaMV 35S promoter and CaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-DXMT
Plasmid#79821PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantPromoterCaMV 35S promoter and CaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only