We narrowed to 5,602 results for: pCas

1. pMZ827

   Plasmid

   #155321

   Purpose

   Constitutive expression of E-PIF6(1-100) in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-E-PIF6(1-100)-NLS-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROF051

   Plasmid

   #155328

   Purpose

   Constitutive expression of SRDX-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-SRDX-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-ATG13

   Plasmid

   #171413

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ATG13 (ATG13 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TDP43 NOTAG3

   Plasmid

   #28208

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa170-414 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   Promoter

   pPEBV

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Double UP mNeon to mScarlet Rac1-N17

   Plasmid

   #125137

   Purpose

   Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).

   Depositor

   Erik Dent

   Article

   Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

   Insert

   mNeon/mScarletP2A Rac1-N17
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Removed Not1 site in mScarlet. 17th AA in Rac1 ch…

   Promoter

   CAG

   Available since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. CP1028-ABE8e-dimer

   Plasmid

   #138498

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459-RhebL1 sgRNA

   Plasmid

   #133769

   Purpose

   Expresses Cas9 and human RhebL1 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. L40C-CRISPR.EFS.PAC

    Plasmid

    #89393

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Use

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFL-mPML-3KRmut-iB

    Plasmid

    #140287

    Purpose

    Expression of mouse PML KR mutant

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    PML (Pml Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330-gp78

    Plasmid

    #61833

    Purpose

    to make gp78 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAYC

    Plasmid

    #62657

    Purpose

    Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT43_GalL_BE-P(AP)3

    Plasmid

    #145036

    Purpose

    Expresses BE-P(AP)3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-(AP)3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO1-puro-U6-sgRNA-TGM2

    Plasmid

    #69239

    Purpose

    U6 driven SpCas9 sgRNA expression for TGM2 site

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TGM2 sgRNA (TGM2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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