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sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-ProteoTuner DD-PuroR

Plasmid

#80964

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

ProteoTuner tag

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-RasS17N

Plasmid

#214820

Purpose

Encoding dominant negative HRas

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative HRas

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rac1T17N

Plasmid

#214821

Purpose

Encoding dominant negative Rac1

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rac1

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rab5S34N

Plasmid

#214822

Purpose

Encoding dominant negative Rab5A

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rab5

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24C #2

Plasmid

#231564

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#2) (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24C #2 (SEC24C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24D

Plasmid

#231565

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24D (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24D (SEC24D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

Plasmid

#203963

Purpose

Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noTA (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the TA domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

Plasmid

#203964

Purpose

Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noHMG (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the HMG domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_PuroR

Plasmid

#212651

Purpose

piggyBac vector to express FLAG-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5delta2CARD

Plasmid

#174225

Purpose

Expresses human MDA5 that lacks 2 CARD domains

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of 2CARD

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_NLuc_FRT-PuroR

Plasmid

#177865

Purpose

Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

NLuc-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_BSD_FRT-PuroR

Plasmid

#177867

Purpose

Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

BSD-based EXSISERS

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_SurfaceHaloTag_FRT-PuroR

Plasmid

#177869

Purpose

Cloning Backbone for Surface-HaloTag-based EXSISERS containing FRT-sites flanked PuroR cassette; clone homology arms via BbsI (or BpiI).

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

Surface-HaloTag-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgfr

Plasmid

#170303

Purpose

A knock-out vector for the mouse Ptgfr

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgfr gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_D429A

Plasmid

#51080

Purpose

mammalian expression of ARAF D429A

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

D429A

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

Plasmid

#168106

Purpose

PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

mCherry-KASH

Use

CRISPR and Cre/Lox

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a eIL15 IRES mNeonGreen-P2A-Puro

Plasmid

#248036

Purpose

Constitutively expresses an engineered version of IL15, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL15

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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