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pCRISPEY-Z3-Hyg

Plasmid

#232101

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

PEmax

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1559

Plasmid

#218590

Purpose

Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

GES

Tags

ePTS1

Expression

Yeast

Mutation

Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and p53(1-85)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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ipYUC2Cas-LT1

Plasmid

#210559

Purpose

Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1Cas-LT1

Plasmid

#210556

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(63)-His6

Plasmid

#212717

Purpose

Bacterial expression of NUbo-E3(63)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-E3(48)-His6

Plasmid

#212714

Purpose

Bacterial expression of NUbo-E3(48)-His6

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

6xHis and NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_C99_sfGFP

Plasmid

#207802

Purpose

Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-C100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch3_sfGFP

Plasmid

#207864

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo8

Plasmid

#203959

Purpose

Cloning plasmid containing the spectinomycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Spectinomycin Resistance Gene

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03R

Plasmid

#198664

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F03L

Plasmid

#198663

Purpose

Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

mCherry

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F02R

Plasmid

#198662

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

linker+GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F02L

Plasmid

#198661

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

linker+GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F01R

Plasmid

#198660

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.F01L

Plasmid

#198659

Purpose

Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

GFP

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02R

Plasmid

#198658

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.A02L

Plasmid

#198657

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01R

Plasmid

#198656

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.A01L

Plasmid

#198655

Purpose

Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

Adaptor

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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