We narrowed to 22,189 results for: Sis

1. pDONR-IAA27_no stop

   Plasmid

   #83860

   Purpose

   Gateway donor vector for IAA27 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA27 (PAP2 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR-IAA8_no stop

   Plasmid

   #83874

   Purpose

   Gateway donor vector for IAA8 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR-IAA5_no stop

   Plasmid

   #83868

   Purpose

   Gateway donor vector for IAA5 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA5 (IAA5 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR-IAA3_no stop

   Plasmid

   #83863

   Purpose

   Gateway donor vector for IAA3 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA3 (SHY2 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR-IAA10_no stop

   Plasmid

   #83848

   Purpose

   Gateway donor vector for IAA10 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR-IAA4_no stop

   Plasmid

   #83866

   Purpose

   Gateway donor vector for IAA4 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA4 (ATAUX2-11 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR-IAA15_no stop

   Plasmid

   #83853

   Purpose

   Gateway donor vector for IAA15 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGWB425

   Plasmid

   #74819

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-FLAG MKRN1 S109A

   Plasmid

   #78774

   Purpose

   To overexpress MKRN1 S109A in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   MKRN1 (MKRN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S109A_mutation

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-HA-RIPK3 K89R

    Plasmid

    #78808

    Purpose

    To overexpress -RIPK3 K89R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K89R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HA-RIPK3 K501R

    Plasmid

    #78810

    Purpose

    To overexpress RIPK3 K501R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K501R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-HA-RIPK3 K363R

    Plasmid

    #78809

    Purpose

    To overexpress RIPK3 K363R in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K363R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a_UDP_Xase_E162

    Plasmid

    #73160

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    change glutamate 162 to alanine

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pJK360

    Plasmid

    #71571

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK385

    Plasmid

    #71572

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-KG-mTmod3-150-352_domain

    Plasmid

    #70755

    Purpose

    mouse Tropomodulin3, 150-352 domain for bacterial expression

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Tropomodulin3 150-352 AA fragment (Tmod3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. miR-451 Trap

    Plasmid

    #61487

    Purpose

    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-451 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET151-hUCH37 DM

    Plasmid

    #61931

    Purpose

    Expression of hUCH37 (ISF1) M269E/N291D in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M269E/N291D (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Changed methionine 269 to glutamate and asparagin…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET151-hUCH37 (ISF1)(M148A/F149A)

    Plasmid

    #61933

    Purpose

    Expression of hUCH37 (M148A/F149A) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) (UCHL5 Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Methionine 148 and Phenylalanine 149 were mutated…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

20. MT79

    Plasmid

    #63112

    Purpose

    The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    amiR-ACD6
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR-mCherry-Emi1_ARHGAP5 UTRs

    Plasmid

    #67927

    Purpose

    Expresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-Emi1 (FBXO5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pICSL11062

    Plasmid

    #68256

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_AtU6-26 + sgRNA_BolC.GA4a-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NET33 pEGFP-N2 (594)

    Plasmid

    #61987

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

24. CFP(1-158) in pcDNAI/Amp

    Plasmid

    #60895

    Purpose

    This vector attaches CFP(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    CFP(1-158)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEW-Venus-Sec16A-deltaN

    Plasmid

    #66605

    Purpose

    Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

    Depositor

    David Stephens

    Article

    Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

    Insert

    Sec16A (SEC16A Human)
    

    Use

    Tags

    Venus (EYFP derivative)

    Expression

    Mammalian

    Mutation

    encodes amino acids 1107-1217 of human Sec16A

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Triple mutant

    Plasmid

    #63719

    Purpose

    Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Triple mutant
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

27. CC6

    Plasmid

    #63718

    Purpose

    Voltage sensing domain of Ciona with the D136S/R168H/T193P/V220R mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC6
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

28. CC5

    Plasmid

    #63717

    Purpose

    Voltage sensing domain of Ciona with the D129N/D164N/G187A/V220T mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC5
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

29. CC4

    Plasmid

    #63716

    Purpose

    Voltage sensing domain of Ciona with the D129S/R168A/D186S/R229I mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC4
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

30. CC2

    Plasmid

    #63714

    Purpose

    Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC2
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

31. CC3

    Plasmid

    #63715

    Purpose

    Voltage sensing domain of Ciona with the G122A/D164A/D186A/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC3
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pFRT-TODestFLAGHA_RBPMS_K36E_R38E

    Plasmid

    #65095

    Purpose

    Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    change lysine 36 to glutamic acid and arginine 38…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFRT-TODestFLAGHA_RBPMS_R38Q

    Plasmid

    #65094

    Purpose

    Destination vector with RBPMS with R38Q mutation for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed arginine 38 to glutamine

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A

    Plasmid

    #65658

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39A

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 36 to glutamic acid, arginine 38 t…

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A

    Plasmid

    #65657

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, E39A

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed lysine 36 to alanine, arguing 38 to alani…

    Promoter

    CMV, 2x Tet

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. OpenLoopControl

    Plasmid

    #59895

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-myc-hIFITM3-deltaY20

    Plasmid

    #58463

    Purpose

    Expresses human IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 deleted

    Promoter

    CMV IE

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pCMV-HA-mIFITM3-D92N

    Plasmid

    #58412

    Purpose

    Expresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Apartate 92 to Asparagine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pCMV-HA-mIFITM3-K24,88,104A

    Plasmid

    #58406

    Purpose

    Expresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 24, 88, and 104 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pCMV-HA-mIFITM3-N2A

    Plasmid

    #58410

    Purpose

    Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Asparagine 2 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pCMV-HA-mIFITM3-K24,83,104A

    Plasmid

    #58407

    Purpose

    Expresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 24, 83, and 104 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

42. pCMV-HA-mIFITM3-S38N

    Plasmid

    #58411

    Purpose

    Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 38 to Asparagine

    Promoter

    CMV IE

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pCMV-HA-mIFITM3-K24,83,88A

    Plasmid

    #58404

    Purpose

    Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 24, 83, and 88 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

44. pCMV-HA-mIFITM3-K88A

    Plasmid

    #58402

    Purpose

    Expresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 88 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

45. pCMV-HA-mIFITM3-K104A

    Plasmid

    #58403

    Purpose

    Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 104 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

46. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

47. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

48. pVRc02_915

    Plasmid

    #49729

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS02_915
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pVRc37_2513

    Plasmid

    #49751

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS37_2513
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pPK1E-2

    Plasmid

    #48146

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge