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PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

Plasmid

#119312

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-500

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

Plasmid

#119311

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-376

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

Plasmid

#119316

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 481-852

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSN67

Plasmid

#100124

Purpose

expresses putative KLF4 DBD with a 6xHis tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Insert corresponds to the DBD of KLF4

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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CD8a-cNLS-EGFP

Plasmid

#86052

Purpose

A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Mutation

CD8a luminal and transmembrane along with two SV4…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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AcrIIC1X* (AcrIIC1X-mCherry chimera)

Plasmid

#128114

Purpose

Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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NC1 pGLUE WTX (1-804)

Plasmid

#36956

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pCR8-CHD5-FRAGMENT

Plasmid

#68879

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Gateway entry vector

Mutation

331-672aa

Promoter

none

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pLJM5-I560S-mDPYD

Plasmid

#61615

Purpose

The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (Dpyd Mouse)

Expression

Mammalian

Mutation

Changed Isoleucine at position 560 to Serine

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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SP-cTAT-zsGreen-IRES-H2B-tdTomato

Plasmid

#254722

Purpose

Expresses secreted, cell-penetrating form of zsGreen for niche labeling and expresses H2B-tdTomato as nuclear reporter.

Depositor

Judith Agudo

Article

Cassandras et al Nature. 2026 Mar 4. doi: 10.1038/s41586-

Insert

SP-cTAT-zsGreen-IRES-H2B-tdTomato

Expression

Bacterial and Mammalian

Promoter

PGK

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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huCofilin K22Q SSR-mRFP

Plasmid

#252617

Purpose

Expresses shRNA resistant human cofilin with K22Q mutation that reduces nuclear uptake and serves as a cofilactin rod reporter

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q; silent mutations at nt 67 to 75 in which TC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Brd9(mouse)-3HA

Plasmid

#248780

Purpose

Express mouse Brd9 in mammalian cells

Depositor

Wenyan Ren

Article

Wu et al Commun Biol. 2025 Dec 1. doi: 10.1038/s4

Insert

brd9 (Brd9 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1

Plasmid

#249270

Purpose

Expression of GLI1

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA + 3xGS linker

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA_V129E

Plasmid

#249269

Purpose

Expression of GPR161 V129E mutant

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Mutation

V129E

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL2809

Plasmid

#232510

Purpose

Expression plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2820

Plasmid

#232511

Purpose

Expression plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16167 AU1.Flag.Ollas

Plasmid

#241479

Purpose

AU1.Flag.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Flag, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16170 AU1.FLAG.V5

Plasmid

#241480

Purpose

AU1.FLAG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, FLAG, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16173 AU1.HA.Ollas

Plasmid

#241481

Purpose

AU1.HA.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16186 AU1.Ollas.S

Plasmid

#241484

Purpose

AU1.Ollas.S Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Ollas, and S

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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