We narrowed to 14,039 results for: TIM

1. pCMV5-NUP98n-CAGEn-NES (Nrdj1)

   Plasmid

   #241418

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   NUP98n-CAGEn-NES (Nrdj1) (NUP98 Synthetic, Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Calmodulin1 (CaM)

   Plasmid

   #240202

   Purpose

   Calmodulin-1 for CaMK2-alpha sensor assays in mammalian cell lines

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   calmodulin-1 (Calm1 Mouse)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   July 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TRE-ETV2

   Plasmid

   #225051

   Purpose

   PiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Ding et al Biotechnol Bioeng. 2025 Jul;122(7):1914-

   Insert

   ETS Variant Transcription Factor 2 (ETV2 Human)
   

   Expression

   Mammalian

   Mutation

   Codon Optimized

   Promoter

   TRE3G

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas3-CDA1

   Plasmid

   #229534

   Purpose

   pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   Cas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP-seq-copGFP-v2-no-scaffold

   Plasmid

   #228520

   Purpose

   CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

   Depositor

   Jellert Gaublomme

   Article

   Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 CAPN7 (1-75)

   Plasmid

   #193031

   Purpose

   Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 1-75

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CAPN7 (81-165)

   Plasmid

   #193032

   Purpose

   Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 81-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABEmax-Puro V2

   Plasmid

   #226955

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-hU6-crBACH1-array_EF1a-BFP

   Plasmid

   #224787

   Purpose

   BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBACH1-1, crBACH1-2, crBACH1-3,
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUt-mNF-Cas13d

    Plasmid

    #224789

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-d2i

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LPUtopia-7_CoV-2

    Plasmid

    #224782

    Purpose

    Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    partial RdRp and N gene (N, ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid

    #169135

    Purpose

    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Coh7
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28:LanIV-Chondromyces

    Plasmid

    #225078

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Chondromyces)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28:LanI-101

    Plasmid

    #225065

    Purpose

    Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanI-101)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCX-CD28 Y189F

    Plasmid

    #223641

    Purpose

    Retroviral expression of mouse CD28 (Y189F) in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (Cd28 Mouse)
    

    Use

    Retroviral; mammalian expression

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-CD80-CLIP

    Plasmid

    #223590

    Purpose

    Lentiviral expression of human CD80 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-SNAP-CD80

    Plasmid

    #223588

    Purpose

    Lentiviral expression of human CD80 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-C1-FKBPflex-ELL-CAAX

    Plasmid

    #220076

    Purpose

    an extra long linker attached to a CAAX domain with an mCherry tag

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    CAAX (HRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid

    #216116

    Purpose

    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G_LF(C199S)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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