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YFP-DAT

Plasmid

#90228

Purpose

Expresses human DAT in mammalian cells

Depositor

Alexander Sorkin

Publication

Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-Rab8a-TN

Plasmid

#86077

Purpose

A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed threonine 22 to aspargine

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Publication

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Publication

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-mnx1

Plasmid

#82402

Purpose

Gateway-compatible zebrafish mnx1 promoter

Depositor

Judith Eisen

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mnx1 promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pME-mTagBFP-CAAX

Plasmid

#75149

Purpose

Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Publication

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNR220

Plasmid

#79541

Purpose

Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI
A118

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSLC-246

Plasmid

#73190

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Publication

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-tse2

Expression

Bacterial

Promoter

PrhaB

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-WT

Plasmid

#78920

Purpose

Mammalian expression of RNF138 with an EGFP fusion

Depositor

Michael Hendzel

Publication

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pCM18

Plasmid

#78565

Purpose

60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

Depositor

James Kaper

Publication

Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

Type

Empty backbone

Tags

luxCDABE

Expression

Bacterial

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Publication

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

GFP

Expression

Mammalian

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Publication

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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tdTomato-VE-Cadherin-N-10

Plasmid

#58142

Purpose

Localization: Tight Junctions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

VE-Cadherin (CDH5 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

I503T in VE-Cadherin

Promoter

CMV

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
msfGFP-Cx43

Plasmid

#69024

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Publication

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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hV1aR-GFP

Plasmid

#67846

Purpose

mammalian expression of human V1a receptor with GFP tag

Depositor

Christian Gruber

Publication

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

V1a receptor (AVPR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
PM2-FRB-CFP

Plasmid

#67518

Purpose

FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence from human Lyn…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Publication

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles