We narrowed to 6,078 results for: nls

1. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

   Plasmid

   #113692

   Purpose

   U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 (NEWENTRY )
   SaCas9 gRNA Cassette (NEWENTRY )
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129028

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Synthetic, S. aureus, Human)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter;

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

   Plasmid

   #199265

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1062

   Plasmid

   #29011

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple155-Cre/EGFP-NLS
   

   Use

   High copy number homologous recombination plasmid…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-HF1RA-PGK-Puro

   Plasmid

   #110860

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7pr-His6-MBP-TEV-dCas9-BirA*

   Plasmid

   #159994

   Purpose

   Bacterial expression of dCas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   dCas9-BirA*
   

   Use

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PE SpG

    Plasmid

    #179081

    Purpose

    All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-Neuroligin-4* [N98/7R]

    Plasmid

    #140081

    Purpose

    Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/7.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neuroligin-4* (Mouse) recombinant mouse monoclonal antibody (Nlgn4l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113687

    Purpose

    SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid

    #113688

    Purpose

    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV and CRISPR

    Tags

    Flag, HAx2, KASH, and NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ239-RR

    Plasmid

    #108862

    Purpose

    FnCpf1 Gateway entry plasmid with N623R and K687R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N623R and K687R, Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE4566

    Plasmid

    #88904

    Purpose

    Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid

    #140193

    Purpose

    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta), NLS

    Expression

    Mutation

    D832A DNAse deactivated

    Promoter

    PgpdA and PtrpC

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-PE VQR

    Plasmid

    #162797

    Purpose

    All-in-one prime editor piggyBac transposon, VQR variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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