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pX330_SYCP3_nterm

Plasmid

#222524

Purpose

Cas9/sgRNA plasmid for targeting SYCP3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVA.009

Plasmid

#37972

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

pJRTCLCrVA.009

Use

Agrobacterium binary

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

3xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHDR_REC8-T2A-mGreenLantern-puroTK

Plasmid

#222521

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to REC8.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Hyg

Plasmid

#100559

Purpose

hgRNA-A21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZCWPW1

Plasmid

#222543

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZCWPW1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ZCWPW1 (ZCWPW1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MSX1

Plasmid

#222557

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MSX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MSX1 (MSX1 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-soNadV

Plasmid

#83363

Purpose

Expresses nadV from Shewanella oneidensis in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Insert

nadV

Expression

Bacterial

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-mScl

Plasmid

#64895

Purpose

Lentiviral expression of Flag-tagged murine transcription factor Scl

Depositor

Georges Lacaud

Article

Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

Insert

Scl

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pStrep-2xEGFP-N1

Plasmid

#122489

Purpose

Expression of Strep-tagged 2xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 2xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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hgRNA-F21_pLKO-Hyg

Plasmid

#100564

Purpose

hgRNA-F21 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-F21

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p12xEGFP-N1

Plasmid

#122172

Purpose

Expression of 12xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

12xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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