We narrowed to 5,937 results for: plasmid dna

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SpyTag-RBD

Plasmid

#159999

Purpose

Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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optGluCl alpha EYFP

Plasmid

#15104

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl alpha (glc-1 Nematode)

Tags

EYFP

Expression

Mammalian

Mutation

Codon optimized cDNA

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/20]

Plasmid

#206744

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/44]

Plasmid

#206743

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/8]

Plasmid

#199429

Purpose

Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targetingGFP (Aequorea victoria)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL4D-V5-His6

Plasmid

#67447

Purpose

Mammalian expression of hArl4D with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Tags

His and V5

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4D-GFP

Plasmid

#67405

Purpose

Mammalian expression of hArl4D with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
hHSF1 RNAi resistant

Plasmid

#118347

Purpose

This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

RNAi resistance mutations (does not change amino …

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag-Cas9

Plasmid

#228958

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLXIN2-RFP

Plasmid

#99205

Purpose

RFP expressing bicistronic retroviral vector

Depositor

Saroj Mathupala

Insert

Red fluorescent protein

Use

Retroviral; Bicistronic retroviral expression vec…

Expression

Mammalian

Mutation

The dsRed cDNA was PCR amplified off AddGene plas…

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1365 LV EF1a-CD28 IRES-EGFP

Plasmid

#201921

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD28 (CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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